Selective NPY (Y5) antagonists (tricyclics)

ABSTRACT

This invention is directed to tricyclic compounds which are selective antagonists for NPY (Y5) receptors. The invention provides a pharmaceutical composition comprising a therapeutically effective amount of the compound of the invention and a pharmaceutically acceptable carrier. This invention provides a pharmaceutical composition made by combining a therapeutically effective amount of the compound of this invention and a pharmaceutically acceptable carrier. This invention further provides a process for making a pharmaceutical composition comprising combining a therapeutically effective amount of the compound of the invention and a pharmaceutically acceptable carrier.

BACKGROUND OF THE INVENTION

Throughout this application, various references are referred to within parentheses. Disclosures of these publications in their entireties are hereby incorporated by reference into this application to more fully describe the state of the art to which this invention pertains. Full bibliographic citation for these references may be found at the end of this application, preceding the claims.

The peptide neurotransmitter neuropeptide Y (NPY) is a 36 amino acid member of the pancreatic polypeptide family with widespread distribution throughout the mammalian nervous system (Dumont et al., 1992). The family includes the pancreatic polypeptide (PP), synthesized primarily by endocrine cells in the pancreas; peptide YY (PYY), synthesized primarily by endocrine cells in the gut; and NPY, synthesized primarily in neurons (Michel, 1991; Dumont et al., 1992; Wahlestedt and Reis, 1993). All pancreatic polypeptide family members share a compact structure involving a "PP-fold" and a conserved C-terminal hexapeptide ending in Tyr³⁶ (or Y³⁶ in the single letter code). The striking conservation of Y³⁶ has prompted the reference to the pancreatic polypeptides' receptors as "Y-type" receptors (Wahlestedt et al., 1987), all of which are proposed to function as seven transmembrane-spanning G protein-coupled receptors (Dumont et al., 1992). NPY and its relatives elicit a broad range of physiological effects through activation of at least five G protein-coupled receptor subtypes known as Y1, Y2, Y3, Y4 (or PP), and the "atypical Y1". While the Y1, Y2, Y3, and Y4 (or PP) receptors were each described previously in both radioligand binding and functional assays, the "atypical Y1" receptor is unique in that its classification is based solely on feeding behavior induced by various peptides including NPY.

The role of NPY in normal and abnormal eating behavior, and the ability to interfere with NPY-dependent pathways as a means to appetite and weight control, are areas of great interest in pharmacological and pharmaceutical research (Sahu and Kalra, 1993; Dryden et al., 1994). NPY is considered to be the most powerful stimulant of feeding behavior yet described (Clark et al., 1984; Levine and Morley, 1984; Stanley and Leibowitz, 1984). The stimulation of feeding behavior by NPY is thought to occur primarily through activation of the hypothalamic "atypical Y1" receptor. For example, direct injection of NPY into the hypothalamus of satiated rats can increase food intake up to 10-fold over a 4-hour period (Stanley et al., 1992). Similar studies using other peptides has resulted in a pharmacologic profile for the "atypical Y1" receptor according to the rank order of potencies of peptides in stimulating feeding behavior as follows: NPY₂₋₃₆ ≧NPY˜PYY˜[Leu³¹, Pro³⁴ ]NPY>NPY₁₃₋₃₆ (Kalra et al., 1991; Stanley et al., 1992). The profile is similar to that of a Y1-like receptor except for the anomalous ability of NPY₂₋₃₆ to stimulate food intake with potency equivalent or better than that of NPY. A subsequent report in J. Med. Chem. by Balasubramaniam and co-workers (1994) showed that feeding can be regulated by [D-Trp³² ]NPY. While this peptide was presented as an NPY antagonist, the published data at least in part support a stimulatory effect of [D-Trp³² ]NPY on feeding. In contrast to other NPY receptor subtypes, the "feeding" receptor has never been characterized for peptide binding affinity in radioligand binding assays.

This problem has been addressed by cloning rat and human cDNAs which encode a single receptor protein, referred to herein as Y5, whose pharmacologic profile links it to the "atypical Y1" receptor. The identification and characterization of a single molecular entity which explains the "atypical Y1" receptor allows the design of selective drugs which modulate feeding behavior (WO 96/16542). It is important to note, though, that any credible means of studying or modifying NPY-dependent feeding behavior must necessarily be highly selective, as NPY interacts with multiple receptor subtypes, as noted above (Dumont et al., 1992).

As used in this invention, the term "antagonist" refers to a compound which binds to, and decreases the activity of, a receptor in the presence of an agonist. In the case of a G-protein coupled receptor, activation may be measured using any appropriate second messenger system which is coupled to the receptor in a cell or tissue in which the receptor is expressed. Some specific but by no means limiting examples of well-known second messenger systems are adenylate cyclase, intracellular calcium mobilization, ion channel activation, guanylate cyclase, and inositol phospholipid hydrolysis. Conversely, the term "agonist" refers to a compound which binds to, and increases the activity of, a receptor as compared with the activity of the receptor in the absence of any agonist.

In order to test compounds for selective binding to the human Y5 receptor the cloned cDNAs encoding both the human and rat Y2 and Y4 (or PP) receptors have been used. The human and rat Y5 receptors are described in coassigned U.S. Pat. No. 5,602,024 and in PCT International Application PCT/US95/15646, published Jun. 6, 1996, as WO 96/16542, the contents of which are hereby incorporated by reference into this application The human and rat Y2 receptors are described in coassigned U.S. Pat. No. 5,545,549 and in PCT International Application PCT/US95/01469, published Aug. 10, 1995, as WO 95/21245, the contents of which are hereby incorporated by reference into this application. The human and rat Y4 receptors are described in coassigned U.S. Pat. No. 5,516,653 and in PCT International Application PCT/US94/14436, published Jul. 6, 1995, as WO 95/17906, the contents of which are hereby incorporated by reference into this application. The Y1 receptor has been cloned from a variety of species including human, rat and mouse (Larhammar et al., 1992; Herzog et al., 1992; Eva et al., 1990; Eva et al., 1992).

Using the NPY-Y5-selective antagonist CGP 71683A, it was demonstrated recently that food intake in free-feeding and energy-derived lean rats is mediated by the Y5 receptor (Criscione et al., 1998). CGP 71683A has high affinity for the cloned rat NPY-Y5 receptor subtype, but 1,000-fold lower affinity for the cloned rat NPY-Y1, Y2, and Y4 receptors. Examples of additional NPY-Y5-selective compounds are described in WO 97/20823, WO 98/35957, and WO 98/35944.

In one embodiment of this invention the synthesis of novel tricyclic compounds which bind selectively to the cloned human Y5 receptor, compared to the other cloned human NPY receptors, and inhibit the activation of the cloned human Y5 receptor as measured in in vitro assays is disclosed. The in vitro receptor binding and activation assays described hereinafter were performed using various cultured cell lines, each transfected with and expressing only a single Y-type receptor.

In addition, the compounds of the present invention may also be used to treat abnormal conditions such as feeding disorders (obesity and bulimia nervosa), sexual/reproductive disorders, depression, epileptic seizure, hypertension, cerebral hemorrhage, congestive heart failure, sleep disturbances, or any condition in which antagonism of a Y5 receptor may be beneficial.

SUMMARY OF THE INVENTION

The invention provides a compound having the structure: ##STR1## wherein each R₁ is independently H, F, Cl, Br, --CN, --OH, --NO₂, --NR₅ R₆, --SO₂ R₅, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) NR₅ COR₅, perfluoroalkyl, polyfluoroalkyl, aminoalkyl, or straight chained or branched C₁ -C₇ alkyl;

wherein R₅ is independently H; or straight chained or branched C₁ -C₇ alkyl;

wherein R₆ is independently H; or straight chained or branched C₁ -C₇ alkyl;

wherein B is O, NH or S;

wherein X is S, SO or SO₂ ;

wherein each n independently is an integer from 0 to 6 inclusive;

wherein R₈ is ##STR2## wherein Y is C or N; wherein R₇ is independently straight chained or branched C₁ -C₇ alkyl;

wherein R₉ is independently H; or straight chained or branched C₁ -C₄ alkyl;

wherein R₁₀ is independently H; or straight chained or branched C₁ -C₄ alkyl;

wherein R₁₁ is ##STR3## wherein R₁₂ is H, straight chained or branched C₁ -C₇ alkyl, (CH₂)_(u) OR₁₇, or O(CH₂)_(u) OR₁₇ ; provided that when X is O, R₁₂ cannot be methyl;

wherein R₁₃ is independently H; --(CH₂)_(u) OR₅ ; --(CH₂)_(t) CONR₅ R₆ ; --(CH₂)_(u) NR₅ COR₅ ; --(CH₂)_(t) COR₇ ; --(CH₂)_(t) CO₂ R₅ ; --(CH₂)_(u) NR₅ R₆ ; --(CH₂)_(u) CN; straight chained or branched C₁ -C₇ alkyl; C₁ -C₇ alkyl in which the C₂ -C₇ atoms may be optionally substituted with one or more F or Cl; C₃ -C₇ cycloalkyl-C₁ -C₇ alkyl; straight chained or branched C₂ -C₇ alkenyl or alkynyl; or C₃ -C₇ cycloalkyl; phenyl or C₁ -C₆ phenylalkyl; wherein the phenyl or C₁ -C₆ phenylalkyl may be substituted with one or more of F, Cl, --CN, --NO₂, --NR₅ R₆, --SO₂ R₅, --(CH₂)_(n) COR₇, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) NR₅ COR₅, --(CH₂)_(n) CO₂ R₅, --(CH₂)_(n) SO₂ NR₅ R₆, straight chained or branched C₁ -C₇ alkyl, perfluoroalkyl, polyfluoroalkyl, or aminoalkyl;

or R₁₂ and R₁₃ together with the amide linkage to which they are attached are pyrrolidinonyl, piperidonyl, or oxazolidinonyl;

wherein R₁₄ is H; straight chained or branched C₁ -C₄ alkyl; F; or --(CH₂)_(r) OR₅ ;

wherein R₁₅ is H, straight chained or branched C₁ -C₄ alkyl, or F;

with the proviso that when R₁₄ is --OH, R₁₅ cannot be F;

wherein R₁₆ is perfluoroalkyl, unsubstituted straight chained or branched C₁ -C₇ alkyl, substituted straight chained or branched C₂ -C₇ alkyl, wherein the C₂ -C₇ alkyl may be substituted with one or more of F, Cl, --CN, --SO₂ R₅, --(CH₂)_(n) COR₇, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) NR₅ COR₅, --(CH₂)_(n) CO₂ R₅, --(CH₂)_(n) OCF₃, perfluoroalkyl, polyfluoroalkyl, or aminoalkyl, straight chained or branched C₂ -C₇ alkenyl or alkynyl, or C₃ -C₇ cycloalkyl or cycloalkenyl; C₃ -C₇ cycloalkyl or cycloalkenyl; phenyl, heteroaryl, or C₁ -C₇ phenylalkyl, wherein the phenyl, heteroaryl, or C₁ -C₇ phenylalkyl may be substituted with one or more of F, Cl, Br, --CN, --NO₂, --NR₅ R₆, --(CH₂)_(n) NR₅ COR₅, --SO₂ R₅, --(CH₂)_(n) COR₇, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) CO₂ R₅, --(CH₂)_(n) SO₂ NR₅ R₆, ethylenedioxy, methylenedioxy, straight chained or branched C₁ -C₇ alkyl, perfluoroalkyl, polyfluoroalkyl, or aminoalkyl, straight chained or branched C₂ -C₇ alkenyl or alkynyl, or C₃ -C₇ cycloalkyl or cycloalkenyl; quinolinyl, 1-naphthyl, 2-naphthyl, or 2,1,3-benzothiadiazolyl; wherein the quinolinyl, 1-naphthyl, 2-naphthyl or 2,1,3-benzothiadiazolyl may be substituted with one or more of F, Cl, Br, --CN, --NO₂, --NR₅ R₆, --(CH₂)_(n) NR₅ COR₅, --SO₂ R₅, --(CH₂)_(n) COR₇, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) CO₂ R₅, --(CH₂)_(n) SO₂ NR₅ R₆, ethylenedioxy, methylenedioxy, straight chained or branched C₁ -C₇ alkyl, perfluoroalkyl, polyfluoroalkyl, or aminoalkyl;

with the proviso that when R₈ is NR₉ (R₁₄ R₁₅)_(s) NR₁₀ R₁₁, R₁₆ cannot be quinolinyl;

wherein R₁₇ is H, straight chained or branched C₁ -C₄ alkyl, perfluoroalkyl, or polyfluoroalkyl;

wherein R₁₉ is --(CH₂)_(u) OR₅, --NR₅ R₆, phenyl, or heteroaryl, wherein the phenyl or heteroaryl may be substituted with one or more of F, Cl, Br, --CN, --NO₂, --NR₅ R₆, --(CH₂)_(n) NR₅ COR₅, --SO₂ R₅, --(CH₂)_(n) COR₇, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) CO₂ R₅, --(CH₂)_(n) SO₂ NR₅ R₆, ethylenedioxy, methylenedioxy, straight chained or branched C₁ -C₇ alkyl, perfluoroalkyl, polyfluoroalkyl, or aminoalkyl, straight chained or branched C₂ -C₇ alkenyl or alkynyl, or C₃ -C₇ cycloalkyl or cycloalkenyl;

wherein m is 0 or 1;

wherein each p independently is an integer from 0 to 2 inclusive;

wherein each r independently is an integer from 0 to 3 inclusive;

wherein each s independently is an integer from 1 to 6 inclusive;

wherein t is an integer from 1 to 4 inclusive;

wherein each u independently is an integer from 2 to 4 inclusive;

wherein v is 1 or 2;

with the proviso that when v is 2, m is 0;

wherein z is an integer from 2 to 7;

or a pharmaceutically acceptable salt thereof.

The invention also provides a pharmaceutical composition comprising a therapeutically effective amount of the compound of the invention and a pharmaceutically acceptable carrier. This invention further provides a pharmaceutical composition made by combining a therapeutically effective amount of the compound of this invention and a pharmaceutically acceptable carrier. This invention further provides a process for making a pharmaceutical composition comprising combining a therapeutically effective amount of the compound of the invention and a pharmaceutically acceptable carrier.

DETAILED DESCRIPTION OF THE INVENTION

The invention provides a compound having the structure: ##STR4## wherein each R₁ is independently H, F, Cl, Br, --CN, --OH, --NO₂, --NR₅ R₆, --SO₂ R₅, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) NR₅ COR₅, perfluoroalkyl, polyfluoroalkyl, aminoalkyl, or straight chained or branched C₁ -C₇ alkyl;

wherein R₅ is independently H; or straight chained or branched C₁ -C₇ alkyl;

wherein R₆ is independently H; or straight chained or branched C₁ -C₇ alkyl;

wherein B is O, NH or S;

wherein X is S, SO or SO₂ ;

wherein each n independently is an integer from 0 to 6 inclusive;

wherein R₈ is ##STR5## wherein Y is C or N; wherein R₇ is independently straight chained or branched C₁ -C₇ alkyl;

wherein R₉ is independently H; or straight chained or branched C₁ -C₄ alkyl;

wherein R₁₀ is independently H; or straight chained or branched C₁ -C₄ alkyl;

wherein R₁₁ is ##STR6## wherein R₁₂ is H, straight chained or branched C₁ -C₇ alkyl, (CH₂)_(u) OR₁₇, or O(CH₂)_(u) OR₁₇ ; provided that when X is O, R₁₂ cannot be methyl;

wherein R₁₃ is independently H; --(CH₂)_(u) OR₅ ; --(CH₂)_(t) CONR₅ R₆ ; --(CH₂)_(u) NR₅ COR₅ ; --(CH₂)_(t) COR₅ ; --(CH₂)_(t) CO₂ R₅ ; --(CH₂)_(u) NR₅ R₆ ; --(CH₂)_(u) CN; straight chained or branched C₁ -C₇ alkyl; C₁ -C₇ alkyl in which the C₂ -C₇ atoms may be optionally substituted with one or more F or Cl; C₃ -C₇ cycloalkyl-C₁ -C₇ alkyl; straight chained or branched C₂ -C₇ alkenyl or alkynyl; or C₃ -C₇ cycloalkyl; phenyl or C₁ -C₆ phenylalkyl; wherein the phenyl or C₁ -C₆ phenylalkyl may be substituted with one or more of F, Cl, --CN, --NO₂, --NR₅ R₆, --SO₂ R₅, --(CH₂)_(n) COR₇, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) NR₅ COR₅, --(CH₂)_(n) CO₂ R₅, --(CH₂)_(n) SO₂ NR₅ R₆, straight chained or branched C₁ -C₇ alkyl, perfluoroalkyl, polyfluoroalkyl, or aminoalkyl;

or R₁₂ and R₁₃ together with the amide linkage to which they are attached are pyrrolidinonyl, piperidonyl, or oxazolidinonyl;

wherein R₁₄ is H; straight chained or branched C₁ -C₄ alkyl; F; or --(CH₂)_(r) OR₅ ;

wherein R₁₅ is H, straight chained or branched C₁ -C₄ alkyl, or F;

with the proviso that when R₁₄ is --OH, R₁₅ cannot be F;

wherein R₁₆ is perfluoroalkyl, unsubstituted straight chained or branched C₁ -C₇ alkyl, substituted straight chained or branched C₂ -C₇ alkyl, wherein the C₂ -C₇ alkyl may be substituted with one or more of F, Cl, --CN, --SO₂ R₅, --(CH₂)_(n) COR₇, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) NR₅ COR₅, --(CH₂)_(n) CO₂ R₅, --(CH₂)_(n) OCF₃, perfluoroalkyl, polyfluoroalkyl, or aminoalkyl, straight chained or branched C₂ -C₇ alkenyl or alkynyl, or C₃ -C₇ cycloalkyl or cycloalkenyl; C₃ -C₇ cycloalkyl or cycloalkenyl; phenyl, heteroaryl, or C₁ -C₇ phenylalkyl, wherein the phenyl, heteroaryl, or C₁ -C₇ phenylalkyl may be substituted with one or more of F, Cl, Br, --CN, --NO₂, --NR₅ R₆, --(CH₂)_(n) NR₅ COR₅, --SO₂ R₅, --(CH₂)_(n) COR₇, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) CO₂ R₅, --(CH₂)_(n) SO₂ NR₅ R₆, ethylenedioxy, methylenedioxy, straight chained or branched C₁ -C₇ alkyl, perfluoroalkyl, polyfluoroalkyl, or aminoalkyl, straight chained or branched C₂ -C₇ alkenyl or alkynyl, or C₃ -C₇ cycloalkyl or cycloalkenyl; quinolinyl, 1-naphthyl, 2-naphthyl, or 2,1,3-benzothiadiazolyl; wherein the quinolinyl, 1-naphthyl, 2-naphthyl or 2,1,3-benzothiadiazolyl may be substituted with one or more of F, Cl, Br, --CN, --NO₂, --NR₅ R₆, --(CH₂)_(n) NR₅ COR₅, --SO₂ R₅, --(CH₂)_(n) COR₇, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) CO₂ R₅, --(CH₂)_(n) SO₂ NR₅ R₆, ethylenedioxy, methylenedioxy, straight chained or branched C₁ -C₇ alkyl, perfluoroalkyl, polyfluoroalkyl, or aminoalkyl;

with the proviso that when R₈ is NR₉ (R₁₄ R₁₅)_(s) NR₁₀ R₁₁, R₁₆ cannot be quinolinyl;

wherein R₁₇ is H, straight chained or branched C₁ -C₄ alkyl, perfluoroalkyl, or polyfluoroalkyl;

wherein R₁₉ is --(CH₂)_(u) OR₅, --NR₅ R₆, phenyl, or heteroaryl, wherein the phenyl or heteroaryl may be substituted with one or more of F, Cl, Br, --CN, --NO₂, --NR₅ R₆, --(CH₂)_(n) NR₅ COR₅, --SO₂ R₅, --(CH₂)_(n) COR₇, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) CO₂ R₅, --(CH₂)_(n) SO₂ NR₅ R₆, ethylenedioxy, methylenedioxy, straight chained or branched C₁ -C₇ alkyl, perfluoroalkyl, polyfluoroalkyl, or aminoalkyl, straight chained or branched C₂ -C₇ alkenyl or alkynyl, or C₃ -C₇ cycloalkyl or cycloalkenyl;

wherein m is 0 or 1;

wherein each p independently is an integer from 0 to 2 inclusive;

wherein each r independently is an integer from 0 to 3 inclusive;

wherein each s independently is an integer from 1 to 6 inclusive;

wherein t is an integer from 1 to 4 inclusive;

wherein each u independently is an integer from 2 to 4 inclusive;

wherein v is 1 or 2;

with the proviso that when v is 2, m is 0;

wherein z is an integer from 2 to 7;

or a pharmaceutically acceptable salt thereof.

In one embodiment, the compound comprises the (+) enantiomer. In another embodiment, the compound comprises the (-) enantiomer.

In one embodiment, the compound has the structure: ##STR7##

In another embodiment, the compound has the structure: ##STR8##

In still another embodiment, the compound has the structure: ##STR9##

In a further embodiment, the compound has the structure: ##STR10##

In still further embodiments, the compound has the structure selected from the group consisting of: ##STR11##

In another embodiment, the compound has the structure: ##STR12##

In still another embodiment, the compound has the structure: ##STR13##

In the present invention, the term "heteroaryl" is used to include five and six membered unsaturated rings that may contain one or more heteroatoms such as oxygen, sulfur, and nitrogen. Examples of heteroaryl groups include, but are not limited to, furanyl, thienyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, triazolyl, thiadiazolyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, and triazinyl.

In addition the term "heteroaryl" is used to include fused bicyclic ring systems that may contain one or more heteroatoms such as oxygen, sulfur and nitrogen. Examples of such heteroaryl groups include, but are not limited to, indolizinyl, indolyl, isoindolyl, benzo[b]furanyl, benzo[b]thiophenyl, indazolyl, benzimidazolyl, benzthiazolyl, purinyl, imidazo[2,1-b]thiazolyl, quinolinyl, isoquinolinyl, quinolizinyl, and 2,1,3-benzothiazolyl.

Furthermore, any of the heteroaryl groups recited above may be substituted with thienyl, isoxazolyl, or pyridyl.

Included in this invention are pharmaceutically acceptable salts and complexes of all of the compounds described herein. The salts include but are not limited to the acids and bases listed herein. The salts include, but are not limited to the following inorganic acids: hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid and boric acid. The salts include, but are not limited to the following organic acids: acetic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, maleic acid, citric acid, methanesulfonic acid, benzoic acid, glycolic acid, lactic acid and mandelic acid. The salts include, but are not limited to the inorganic base, ammonia. The salts include, but are not limited to the following organic bases: methylamine, ethylamine, propylamine, dimethylamine, diethylamine, trimethylamine, triethylamine, ethylenediamine, hydroxyethylamine, morpholine, piperazine and guanidine. This invention further provides for the hydrates and polymorphs of all of the compounds described herein.

The present invention includes within its scope prodrugs of the compounds of the invention. In general, such prodrugs will be functional derivatives of the compounds of the invention which are readily convertible in vivo into the required compound. Thus, in the present invention, the term "administering" shall encompass the treatment of the various conditions described with the compound specifically disclosed or with a compound which may not be specifically disclosed, but which converts to the specified compound in vivo after administration to the patient. Conventional procedures for the selection and preparation of suitable prodrug derivatives are described, for example, in Design of Prodrugs, ed. H. Bundgaard, Elsevier, 1985.

The present invention further includes metabolites of the compounds of the present invention. Metabolites include active species produced upon introduction of compounds of this invention into the biological milieu.

This invention further provides a pharmaceutical composition comprising a therapeutically effective amount of the compound of the invention and a pharmaceutically acceptable carrier. In one embodiment, the amount of the compound is an amount from about 0.01 mg to about 800 mg. In another embodiment, the amount of the compound is an amount from about 0.01 mg to about 500 mg. In another embodiment, the amount of the compound is an amount from about 0.01 mg to about 250 mg. In another embodiment, the amount of the compound is an amount from about 0.1 mg to about 60 mg. In another embodiment, the amount of the compound is an amount from about 1 mg to about 20 mg. In a further embodiment, the carrier is a liquid and the composition is a solution. In another embodiment, the carrier is a solid and the composition is a tablet. In a further embodiment, the carrier is a gel and the composition is a suppository.

This invention provides a pharmaceutical composition made by combining a therapeutically effective amount of the compound of this invention and a pharmaceutically acceptable carrier.

This invention provides a process for making a pharmaceutical composition comprising combining a therapeutically effective amount of the compound of this invention and a pharmaceutically acceptable carrier.

In the subject invention a "therapeutically effective amount" is any amount of a compound which, when administered to a subject suffering from a disease against which the compounds are effective, causes reduction, remission, or regression of the disease.

In the practice of this invention the "pharmaceutically acceptable carrier" is any physiological carrier known to those of ordinary skill in the art useful in formulating pharmaceutical compositions.

In one preferred embodiment the pharmaceutical carrier may be a liquid and the pharmaceutical composition would be in the form of a solution. In another equally preferred embodiment, the pharmaceutically acceptable carrier is a solid and the composition is in the form of a powder or tablet. In a further embodiment, the pharmaceutical carrier is a gel and the composition is in the form of a suppository or cream. In a further embodiment the compound may be formulated as a part of a pharmaceutically acceptable transdermal patch.

A solid carrier can include one or more substances which may also act as flavoring agents, lubricants, solubilizers, suspending agents, fillers, glidants, compression aids, binders or tablet-disintegrating agents; it can also be an encapsulating material. In powders, the carrier is a finely divided solid which is in admixture with the finely divided active ingredient. In tablets, the active ingredient is mixed with a carrier having the necessary compression properties in suitable proportions and compacted in the shape and size desired. The powders and tablets preferably contain up to 99% of the active ingredient. Suitable solid carriers include, for example, calcium phosphate, magnesium stearate, talc, sugars, lactose, dextrin, starch, gelatin, cellulose, polyvinylpyrrolidine, low melting waxes and ion exchange resins.

Liquid carriers are used in preparing solutions, suspensions, emulsions, syrups, elixirs and pressurized compositions. The active ingredient can be dissolved or suspended in a pharmaceutically acceptable liquid carrier such as water, an organic solvent, a mixture of both or pharmaceutically acceptable oils or fats. The liquid carrier can contain other suitable pharmaceutical additives such as solubilizers, emulsifiers, buffers, preservatives, sweeteners, flavoring agents, suspending agents, thickening agents, colors, viscosity regulators, stabilizers or osmo-regulators. Suitable examples of liquid carriers for oral and parenteral administration include water (partially containing additives as above, e.g. cellulose derivatives, preferably sodium carboxymethyl cellulose solution), alcohols (including monohydric alcohols and polyhydric alcohols, e.g. glycols) and their derivatives, and oils (e.g. fractionated coconut oil and arachis oil). For parenteral administration, the carrier can also be an oily ester such as ethyl oleate and isopropyl myristate. Sterile liquid carriers are useful in sterile liquid form compositions for parenteral administration. The liquid carrier for pressurized compositions can be halogenated hydrocarbon or other pharmaceutically acceptable propellent.

Liquid pharmaceutical compositions which are sterile solutions or suspensions can be utilized by for example, intramuscular, intrathecal, epidural, intraperitoneal or subcutaneous injection. Sterile solutions can also be administered intravenously. The compounds may be prepared as a sterile solid composition which may be dissolved or suspended at the time of administration using sterile water, saline, or other appropriate sterile injectable medium. Carriers are intended to include necessary and inert binders, suspending agents, lubricants, flavorants, sweeteners, preservatives, dyes, and coatings.

The compound can be administered orally in the form of a sterile solution or suspension containing other solutes or suspending agents (for example, enough saline or glucose to make the solution isotonic), bile salts, acacia, gelatin, sorbitan monoleate, polysorbate 80 (oleate esters of sorbitol and its anhydrides copolymerized with ethylene oxide) and the like.

The compound can also be administered orally either in liquid or solid composition form. Compositions suitable for oral administration include solid forms, such as pills, capsules, granules, tablets, and powders, and liquid forms, such as solutions, syrups, elixirs, and suspensions. Forms useful for parenteral administration include sterile solutions, emulsions, and suspensions.

Optimal dosages to be administered may be readily determined by those skilled in the art, and will vary with the particular compound in use, the strength of the preparation, the mode of administration, and the advancement of the disease condition. Additional factors depending on the particular subject being treated will result in a need to adjust dosages, including subject age, weight, gender, diet, and time of administration.

One skilled in the art will readily appreciate that appropriate biological assays will be used to determine the therapeutic potential of the claimed compounds for treating the above noted disorders.

This invention further provides compositions which need not be pharmaceutical as that term is understood in the art. Such compositions comprise a compound in accordance with the subject invention in an amount effective to agonize and/or antagonize a Y5 receptor and a suitable carrier.

Still further, the invention provides a method of agonizing and/or antagonizing a Y5 receptor which comprises contacting the receptor, e.g. in vitro or in vivo, with an amount of a compound of this invention effective to agonize and/or antagonize the receptor.

This invention will be better understood from the Experimental Details which follow. However, one skilled in the art will readily appreciate that the specific methods and results discussed are merely illustrative of the invention as described more fully in the claims which follow thereafter.

EXPERIMENTAL DETAILS AND RESULTS I. Synthetic Methods for Examples

General Procedures Relating to Examples:

For the formation of 2-aminothiazoles from 2-haloketones and thioureas, see, for example, Kearney, P. C., et al., 1998; Di Fabio, R. and Pentassuglia, G., 1998; De Kimpe, N., et al., 1996; Plazzi, P. V., et al., 1995; and Novikova, A. P., 1991.

For the formation of thiazoles from 2-haloketones and thioamides, see, for example, Critcher, D. J. and Pattenden, G., 1996; and Friedman, B. S., et al., 1937.

For the formation of 2-aminoimidazoles from 2-haloketones and guanidines, see, for example, Little, T. L. and Webber, 1994; and Chabaka, L. M., et al., 1994.

For the formation of imidazoles from 2-haloketones and amidines, see, for example, Demchenko, A. M., et al., 1997; and Nagao, Y., et al., 1996.

For the synthesis of 2-aminooxazoles from 2-haloketones and ureas, see, for example, Pathak, V. N., et al., 1993; Crangk, G. and Foulis, M. J., 1971; and Marchetti, E., et al., 1968.

For the formation of oxazoles from 2-haloketones and amides, see, for example, Hammar, W. J. and Rustad, M. A., 1981; and Zhao, Z., et al., 1991.

All reactions were performed under an inert atmosphere (Argon) and the reagents, neat or in appropriate solvents, were transferred to the reaction vessel via syringe and cannula techniques. The parallel synthesis reaction arrays were performed in vials (without an inert atmosphere) using J-KEM heating shakers (Saint Louis, Mo.). Unless stated otherwise all solvents were AR grade and used as supplied. Anhydrous solvents were purchased from Aldrich Chemical Company and used as received. Examples 1-64 described in this patent application were named using ACD/Name program (version 2.51, Advanced Chemistry Development Inc., Toronto, Ontario, M5H2L3, Canada).

¹ H and ¹³ C spectra were recorded at 300 and 75 MHz (QE Plus) with CDCl₃ as solvent (unless otherwise noted) and tetramethylsilane as internal standard. s=singlet; d=doublet; t=triplet; q=quartet; p=pentet; sextet; septet; b=broad; m=multiplet. Elemental analyses were performed by Robertson Microlit Laboratories, Inc. Low-resolution electrospray MS spectra were measured (ESMS, MS) and MH⁺ is reported. Thin-layer chromatography (TLC) was carried out on glass plates precoated with silica gel 60 F₂₅₄ (0.25 mm, EM Separations Tech.). Preparative thin-layer chromatography was carried out on glass sheets precoated with silica gel GF (2 mm, Analtech). Flash column chromatography was performed on Merck silica gel 60 (230-400 mesh). Melting points were determined in open capillary tubes on a Med-Temp apparatus and are uncorrected.

General Procedure for the Synthesis of Benzothiepin-5-ones:

2,3,4,5-Tetrahydro-1-benzothiepin-5-one:

Step 1.

4-(phenylsulfanyl)butanoic acid:

Sodium methoxide (1.2 equivalent) was added to 60 ml of ethanol, in one portion, and the suspension was stirred at room temperature. Thiophenol (1 equivalent) was added to the above suspension and stirred at room temperature for 30 minutes. Butyrolactone (1.1 equivalent) was added to the reaction mixture and the resulting mixture was stirred at reflux temperature for 6 hours, cooled to room temperature and concentrated in vacuo. The resulting solid was washed with 200 ml hexane/ether 2:1, v/v. The solid was suspended into ice cold 2N HCl solution and stirred for 15 minutes. The resulting solid was filtered, washed with 100 ml hexane/ether and dried under reduced pressure at room temperature to give 4-(phenylsulfanyl)butanoic acid as tan solid: 52% yield; ¹ H NMR (CDCl₃) δ 7.32-7.12 (m, 5H), 2.94 (t, 2H, J=7.2 Hz), 2.41 (t, 2H, J=7.2 Hz), 1.85 (p, 2H, J=7.2 Hz); Anal. Calc. For C₁₀ H₁₂ S₁ O₂ : C, 61.22; H, 6.12. Found: C, 61.16; H, 6.28.

A similar procedure was used for the synthesis of 4-(4-fluorophenylsulfanyl)butanoic acid: 60% yield; ¹ H NMR (CDCl₃) δ 7.34 (m, 2H, 7.00 (m, 2H), 2.94 (t, 2H, J=7.2 Hz), 2.51 (t, 2H, J=7.2 Hz), 1.93 (p, 2H, J=7.2 Hz); Anal. Calc. For C₁₀ H₁₁ F₁ S₁ O₂ : C, 56.07; H, 5.14. Found: C, 55.80; H, 5.19.

Step 2.

Benzothiepin-5-ones:

Polyphosphoric acid (6 equivalents) was heated to 80° C. under argon. 4-(Phenylsulfanyl)butanoic acid from the step above, (1 equivalent) was added in portions and the mixture was kept at 100° C. for 2 hours. The reaction mixture was cooled, dropped into ice cold water and extracted with 2×100 ml ethyl acetate. The combined ethyl acetate extracts were washed with 100 ml water, 100 ml saturated sodium bicarbonate, and 100 ml water. The ethyl acetate extract was dried (anhydrous sodium sulfate), filtered and the solvent removed in vacuo to give a tan solid. The solid was dried under vacuum to give 2,3,4,5-tetrahydro-1-benzothiepin-5-one: 52% yield; ¹ H NMR (CDCl₃) δ 7.824 (dd, 1H, J=0.9, 7.5 Hz), 7.45 (dd, 1H, J=0.6, 6.9 Hz), 7.34-7.21 (m, 2H), 3.05 (t, 2H, J=6.6 Hz), 2.97 (t, 2H, J=6.6 Hz), 2.29 (p, 2H, J=6.6 Hz).

The above described procedure was also used to give 7-fluoro-2,3,4,5-tetrahydro-1-benzothiepin-5-one: 60% yield; ¹ H NMR (CDCl₃) δ 7.51 (dd, 1H, J=3.0, 9.3 Hz), 7.41 (dd, 1H, J=8.7, 5.1 Hz), 7.04 (apparent dt, 1H, J=3.0, 4.8 Hz), 3.06 (t, 2H, J-6.6 HZ), 2.96 (t, 2H, J=6.6 Hz), 2.64 (t, 2H, J-6.9 Hz); Anal. Calc. For C₁₀ H₁₀ S₁ O₁ : C, 67.41; H, 5.61. Found: C, 67.48; H, 5.68.

General Procedure for the Synthesis of Bromoketones:

To the solution of the ketone (1 equivalent) in acetic acid, cooled in a water bath, was added bromine (1 equivalent) slowly. The reaction mixture was stirred at room temperature for 3 hours. Solvents were evaporated, the residue was dissolved in dichloromethane and the resultant solution washed with saturated sodium bicarbonate and water and dried over sodium sulfate. Evaporation of the combined decolorized organic phase afforded the desired product as a light yellow oil in more than 80% yield in most cases.

7-Fluoro-2,3,4,5-tetrahydro-1-benzothiepin-5-one was brominated according to the procedure described below to give 4-bromo-7-fluoro-2,3,4,5-tetrahydro-1-benzothiepin-5-one. A similar procedure was also used to brominate 2,3,4,5-tetrahydro-1-benzothiepin-5-one.

4-Bromo-7-fluoro-2,3,4,5-tetrahydro-1-benzothiepin-5-one:

7-Fluoro-2,3,4,5-tetrahydro-1-benzothiepin-5-one (1 equivalent) was dissolved in glacial acetic acid and stirred at room temperature. Bromine (2.5 equivalents) was added to the above mixture dropwise and stirring continued at room temperature for 4 hours. Water was added to the reaction mixture and the mixture was then extracted with 2×25 ml ethyl acetate. The combined ethyl acetate extracts were washed with water, saturated sodium bicarbonate, and water. The combined ethyl acetate extracts were dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to afford a solid which was re-crystallized from ethyl acetate/hexane 1:1 v/v to afford 4-bromo-7-fluoro-2,3,4,5-tetrahydro-1-benzothiepin-5-one: ¹ H NMR (CDCl₃) δ 7.55 (dd, 1H, J=2.7, 9.0 Hz), 7.44 (dd, 1H, J=8.7, 5.1 Hz), 7.11 (Apparent dt, 1H, J=2.7, 4.8 Hz), 5.34 (dd, 1H, J=5.7, 10.2 Hz), 3.20-2.50 (m, 4H).

4-bromo-2,3,4,5-tetrahydro-1-benzothiepin-5-one: ¹ H NMR (CDCl₃) δ 7.83 (d, 1H, J=7.8 Hz), 5.35 (dd, 1H, J=5.7, 10.5 Hz), 3.30-2.50 (m, 4H).

General Procedure for the Synthesis of Boc Protected Thioureas:

A protected diamine such as N-Boc-1,4-diaminobutane or N-Boc-1,5-diaminopentane (1 equivalent) was dissolved in tetrahydrofuran and stirred at room temperature. Benzoyl thioisocyanate (1 equivalent) was added dropwise to the aforementioned solution. The resulting mixture was stirred at room temperature for 24 hours. The solvent was removed under reduced pressure to give a yellow oil. The yellow oil (1 equivalent) from the above step was dissolved in methanol, an aqueous potassium carbonate (3 equivalents) solution was added, and the mixture stirred for 48 hours. Water was added to the reaction mixture, which was then extracted with 2×75 ml ethyl acetate. The combined ethyl acetate extracts were washed with water, dried with anhydrous sodium sulfate, filtered and the solvent removed under reduced pressure to give the desired thiourea.

tert-Butyl 5-[(aminocarbothioyl)amino]pentylcarbamate was obtained as a light yellow wax from tert-butyl 5-{[(benzoylamino)carbothioyl]amino}-pentylcarbamate. ¹ H NMR (CD₃ OD) δ 3.44 (m, 1H), 3.10 (m, 1H), 3.01 (t, 2H, J=6.7 Hz), 1.60-1.31 (m, 6H), 1.41 (s, 9H); 262 (ESMS, MH⁺).

tert-Butyl 5-{[(benzoylamino)carbothioyl]amino}-pentylcarbamate was obtained as a light yellow solid in 79% yield from N-BOC-1,5-diaminopentate and benzoyl isothiocyanate; m.p. 90-93° C.

tert-Butyl 4-[(aminocarbothioyl)amino]butylcarbamate was obtained as a light yellow wax from tert-butyl 4-{[(benzoylamino)carbothioyl]amino}-butylcarbamate. ¹ H NMR (CD₃ OD) δ 3.48 (m, 1H), 3.10 (m, 1H), 3.05 (t, 2H, J=6.5 Hz), 1.60 (m, 4H), 1.42 (s, 9H); 248 (ESMS, MH⁺).

Tert-Butyl 4-{[(benzoylamino)carbothioyl]amino}butylcarbamate was obtained as a light brown oil in 93% yield from N-BOC-1,4-diaminobutane and benzoyl isothiocyanate.

trans-tert-Butyl {4-[(aminocarbothioyl)amino]cyclohexyl}methylcarbamate was obtained as a light yellow wax from trans-tert-butyl (4-{[(benzoylamino)carbothioyl]amino}cyclohexyl)-methylcarbamate. ¹ H NMR (CD₃ OD) δ 3.92 (m, 1H), 2.86 (m, 2H), 2.00 (m, 2H), 1.76 (m, 2H), 1.41 (s, 9H), 1.37 (m, 1H), 1.06 (m, 4H); 288 (ESMS, MH⁺).

trans-tert-Butyl (4-{[(benzoylamino)carbothioyl]amino}cyclohexyl)-methylcarbamate was obtained as a yellow solid in 97% yield from tert-butyl 4-aminocyclohexylmethylcarbamate and benzoyl isothiocyanate.

trans-tert-Butyl 4-Aminocyclohexylmethylcarbamate was obtained in more than 95% yield from hydrogenation of benzyl 4-{[(tert-butoxycarbonyl)amino]methyl}cyclocarbamate.

Benzyl-4-[[[tert-butoxycarbonyl]amino]methyl]cyclohexylcarbamate: To a stirred suspension of 4-[[(tert-butoxycarbonyl)amino]methyl]cyclohexanecarboxylic acid (Maybridge Chemical Co., Ltd.) (45 g) and diphenylphosphoryl azide (44 ml) in toluene (600 ml) was added triethylamine (32 ml) over a period of 20 min whilst maintaining the internal temperature at -10-0° C. The mixture was slowly warmed and then stirred at 70° C. for 4 h. After cooling to 40° C., benzyl alcohol (36 ml) was added and the reaction mixture heated at reflux for 20 h. The cold reaction mixture was washed with water and brine and dried over anhydrous magnesium sulfate. Removal of the solvent and recrystallization of the organic residue from ethyl acetate and diethyl ether gave the title compound, benzyl-4-[[[tert-butoxycarbonyl]amino]methyl]cyclohexylcarbamate as a white solid, m.p. 129-131° C.

trans-tert-Butyl {4-[(aminocarbothioyl)amino]cyclohexyl}carbamate was obtained as a yellow solid from trans-tert-butyl 4-{[(benzoylamino)carbothioyl]amino}cyclohexyl)-carbamate: ¹ H NMR (CD₃ OD) δ 3.94 (m, 1H), 3.30 (m, 1H), 2.00 (m, 2H), 1.90 (m, 2H), 1.41 (s, 9H), 1.26 (m, 4H); 274 (ESMS, MH⁺).

trans-tert-Butyl 4-{[(Benzoylamino)carbothioyl]amino}cyclohexyl)-carbamate was obtained as a white solid in 66% yield from tert-butyl 4-aminocyclohexylcarbamate and benzoyl isothiocyanate.

trans-tert-Butyl 4-aminocyclohexylcarbamate was obtained as a light yellow wax in more than 95% yield by hydrogenation of benzyl 4-[(tert-butoxycarbonyl)amino]cyclohexylcarbamate.

trans-Benzyl 4-{[(aminocarbothioyl)amino]methyl}cyclohexylcarbamate was obtained as a yellow solid in 71% yield from trans-benzyl 4-({[(Benzoylamino) carbothioyl]amino}methyl)-cyclohexylcarbamate; 322 (ESMS, MH⁺).

trans-Benzyl 4-({[(Benzoylamino)carbothioyl]amino}methyl)-cyclohexylcarbamate was obtained as a yellow solid from benzyl 4-(aminomethyl)cyclohexylcarbamate and benzoyl isothiocyanate.

trans-benzyl 4-(aminomethyl)cyclohexylcarbamate was obtained as a white solid in more than 95% yield by stirring benzyl-4-{[(tert-butoxycarbonyl)amino]-methyl}cyclocarbamate in 2N HCl (made from 1:1 of EtOAc and 4N HCl in dioxane).

General Procedure for the Synthesis of the (4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino Template:

A mixture of a bromoketone such as 7-fluoro-2,3,4,5-tetrahydro-1-benzothiepin-5-one (1 equivalent), a thiourea (1 equivalent), and diisopropylethylamine (2 equivalents) in anhydrous ethanol was stirred and heated at reflux temperature overnight. The solvent was evaporated, the brown residue dissolved in dichloromethane and the resultant solution washed with saturated aqueous sodium bicarbonate. The aqueous phase was extracted with dichloromethane three times. The combined extracts were dried over anhydrous sodium sulfate. The crude product was purified by flash column chromatography (Silica Gel, hexanes: ethyl acetate). An example of the aforementioned general procedure follows.

4-Bromo-2,3,4,5-tetrahydro-1-benzothiepin-5-one (1.2 equivalent, 29.76 mmol) and tert-butyl 5-[(aminocarbothioyl)amino]pentylcarbamate (1 equivalent, 24.8 mmol) were mixed with 2 equivalents diisopropylethyl amine in 200 ml of EtOH. The reaction mixture was heated at reflux temperature overnight. The dark brown reaction mixture was concentrated and chromatographed (silica) to obtain tert-butyl-N-{5-[(9-fluoro-4,5-dihydrobenzo[2,3]-thiepino[4,5-d][1,3]thiazol-2-yl)amino]pentyl}-carbamate as a light tan solid.

General Procedure for the Deprotection of BOC-Protected Amines:

tert-butyl N-{[4-(4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}carbamate or tert-butyl N-[6-(4,5-dihydrobenzo[2,3]-thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]carbamate were separately dissolved in Et₂ O. The same volume of 4N HCl in dioxane was added to make a 2N solution. The reaction mixture was stirred at room temperature overnight, and the solvent removed under reduced pressure to obtain the desired product as its HCl salt.

N1-(4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-yl)-1,4-butanediamine: 45% yield; ¹ H NMR (CDCl₃) δ 8.05 (dd, 1H, J=0.56, 8.4 Hz), 7.33 (dd, 1H, J=0.6, 8.4 Hz), 7.26 (t, 1H, J=6.5 Hz), 7.17 (t, 1H, J=6.5 Hz), 5.91 (broad, 1H), 3.20 (m, 6H), 2.69 (t, 2H, J=6.5 Hz), 1.61-1.27 (m, 6H).

N1-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-yl)-1,5-pentanediamine: 50% yield; ¹ H NMR (CDCl₃) δ 8.03 (dd, 1H, J=0.6, 8.4 Hz), 7.49 (dd, 1H, J=0.6, 8.4 Hz), 7.28 (t, 1H, J=6.5 Hz), 7.16 (t, 1H, J=6.5 Hz), 5.92 (broad, 1H), 3.13 (m, 6H), 2.63 (t, 2H, J=6.5 Hz), 1.57-1.37 (m, 8H).

tert-Butyl N-{5-[(9-Fluoro-4,5-dihydrobenzo-[2,3]thiepino[4,5-d][1,3]thiazol-2-yl)amino]pentyl}carbamate: 60% yield; Anal. Calc. for C₂₁ H₂₈ N_(3F) S₂ O₂ +0.15 CH₂ Cl₂ : C, 56.41; H, 6.33; N, 9.3. Found: C, 56.45; H, 6.17; N, 8.9; ¹ H NMR (CDCl₃) δ 7.72 (dd, 1H, J=1.15, 7.5 Hz), 7.47-7.04 (m, 1H), 6.89-6.83 (m, 1H), 6.190-6.142 (m, 1H), 4.747-4.690 (m, 1H), 3.370-2.803 (m, 8H), 1.64-1.048 (m, 6H), 1.407 (s, 9H).

N2-[4-(Aminomethyl)cyclohexyl]-4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-amine: 73% yield, 346 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.05 (dd, 1H, J=1.2, 7.9 Hz), 7.50 (dd, 1H, J=1.2, 7.7 Hz), 7.32 (apparent dt, 1H, J=1.8, 7.2 Hz), 7.15 (apparent dt, 1H, J=1.7, 7.2 Hz), 4.93 (b, 1H), 3.23 (m, 1H), 2.99 (t, 2H, J=6.3 Hz), 2.56 (d, 2H, J=6.6 Hz), 2.04 (ABM, 4H), 1.70-0.80 (m, 12H).

tert-Butyl N-[6-(4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]carbamate: 51% yield, 434 (ESMS, MH⁺); ¹ H NMR (CD₃ OD) δ 7.92 (d, 1H, J=7.5 Hz), 7.48 (d, 1H, J=7.6 Hz), 7.30 (apparent dt, 1H, J=1.2, 7.7 Hz), 7.15 (apparent dt, 1H, J=1.5, 7.5 Hz), 3.30(t, 2H, J=1.6 Hz), 3.16 (t, 2H, J=6.3 Hz), 3.05 (t, 2H, J=5.9 Hz), 3.01 (t, 2H, J=6.5 Hz), 1.63 (m, 2H), 1.42 (s, 9H), 1.51-1.28 (m, 6H).

N1-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-yl)-1,6-hexanediamine: 75% yield, 334 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.05 (dd, 1H, J=1.0, 8.1 Hz), 7.51 (dd, 1H, J=1.1, 7.8 Hz), 7.32 (apparent dt, 1H, J=1.4, 7.4 Hz), 7.15, (apparent dt, 1H, J=1.6, 7.6 Hz), 5.15 (broad, 1H), 3.23(m, 4H), 3.19 (s, 2H), 2.68 (t, 2H, J=5.7 Hz), 1.70-1.21 (m, 8H).

tert-Butyl N-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}carbamate: 44% yield, 446 (ESMS, MH⁺); ¹ H NMR (CD₃ OD) δ 7.90 (dd, 1H, J=1.2, 7.8 Hz), 7.49 (dd, 1H, J=0.8, 7.8 Hz), 7.32 (apparent dt, 1H, J=1.4, 7.7 Hz), 7.16 (apparent dt, 1H, J=1.3, 7.6 Hz), 3.41 (m, 1H), 3.30 (m, 2H), 3.19 (t, 2H, J=6.5 Hz), 3.06, (t, 2H, J=5.8 Hz), 2.90 (d, 2H, J=7.0 Hz), 1.99 (ABm, 4H), 1.43 (s, 9H), 1.32-1.05 (m, 3H).

General Procedure for the Derivatization of Amines with Carboxylic Acid and Sulfonic Acid Derivatives:

An amine such as N1-(4,5-dihydrobenzo-[2,3]thiepino[4,5-d][1,3]thiazol-2-yl)-1,6-hexanediamine or N2-[4-(Aminomethyl)cyclohexyl]-4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-amine (0.305 mmol) was dissolved in 2 ml CH₂ Cl₂ containing 2 equivalents of diisopropylethylamine. A sulfonyl or acid chloride (1-3 equivalents) was added dropwise. The reaction mixture was stirred at room temperature for 1-3 days, quenched with water, washed with 10% NaHCO₃, dried over Na₂ SO₄ and chromatographed using column chromatography or preparative TLC.

General Procedure for the Derivatization of Tricyclic Amino Template such as N1-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-yl)-1,6-hexanediamine Using Parallel Synthesis:

Tricyclic amine templates such as N1-(4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-yl)-1,6-hexanediamine (1 equivalent) or N2-[4-(aminomethyl)cyclohexyl]-4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-amine (1 equivalent), contained in a Robbins Scientific FlexChem 96-well assay, were treated with dichloromethane and poly(4-vinylpyridine). The required sulfonyl chloride, acid chloride, isocyanate or carbamyl chloride (1 equivalent) was added to each well. The reaction plates were rotated in a Robbins Scientific FlexChem rotating oven at room temperature for 24 hours, the contents filtered into a second reaction plate, and dichloromethane and polymer-supported tris(2-aminoethyl)amine were added. The second FlexChem plate was rotated at room temperature for an additional 24 hours. The contents were then filtered through a silica gel pad contained in a third Robbins plate and the filtrate collected in a 96-deep well plate. The wells were eluted with hexanes followed by EtOAc and a mixture of EtOAc:MeOH=8:2. The solvent was removed and the crude products screened for affinity at hY5 (single point, 100 nM). Compounds exhibiting more than 50% inhibition were chromatographed for full pharmacological evaluation.

General Procedure for the Formation of Formamides:

tert-Butyl-N-[4-(Isopropylamino)cyclohexyl]methyl-carbamate:

Isopropyl iodide (2 equivalents) was added dropwise to a suspension of tert-butyl N-[4-aminocyclohexyl]methylcarbamate (1 equivalent, [229 (ESMS, MH⁺): ¹ H NMR (CD₃ OD) δ 3.33 (m, 1H), 3.29 (m, 2H), 2.85 (d, 2H, J=6.4 Hz), 2.57 (m, 1H), 1.80 (ABm, 4H), 1.41 (s, 9H), 1.35 (m, 1H), 1.20-0.88 (m, 4H)]) and diisopropylethyl amine (3 equivalents) in THF. The resulting mixture was stirred for 1 day. TLC analysis showed some starting amine. Isopropyl iodide (1 equivalent) and diisopropylethyl amine (3 equivalents) were added to the reaction mixture which was then heated at 40° C. for 1 day. The reaction mixture was concentrated and chromatrographed to give tert-butylN-[4-(isopropylamino)cyclohexyl]methyl carbamate: 22% yield, 271 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 4.65 (broad, 1H), 2.91 (m, 3H), 2.42 (m, 1H), 1.80 (ABm, 4H), 1.38 (s, 9H), 0.98 (d, 6H, J=6.3 Hz), 1.32-0.85 (m, 5H).

tert-Butyl-N-[4-(2-methoxyethylamino)-cyclohexyl]methylcarbamate was similarly obtained (2-methoxyethyl bromide and n-Bu₄ NI were used): 35% yield, 378 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 4.64 (broad, 1H), 3.44 (m, 2H), 3.31 & 3.30 (two s, 3H), 2.92 (m, 2H), 2.74 (m, 2H), 2.33 (m, 1H), 1.81 (ABm, 4H), 1.39 & 1.38 (two s, 9H), 1.34 (m, 1H), 0.98 (m, 4H).

tert-Butyl-N-[4-(isopropylformylamino)cyclohexyl]-methylcarbamate:

A solution of a tert-butyl N-[4-(isopropylamino)-cyclohexyl]methylcarbamate (7.89 mmol, 1 equivalent) in THF (5 ml) was added dropwise to a solution of 1H-benzotriazole-1-carboxaldehyde (8.68 mmol, 1.2 equivalent) in THF (10 ml) at room temperature, stirred overnight and heated at reflux temperature for two hours. 1H-Benzotriazole-1-carboxaldehyde (1 equivalent) was added and stirred overnight. The solvent was removed and dichloromethane was added to the residue. The organic extract was washed with 2N NaOH solution, washed with saturated NaCl solution, and dried over Na₂ SO₄. The solvent was then removed and the product chromatographed to give tert-butyl N-[4-(isopropylformylamino)cyclohexyl]methylcarbamate: 100% yield, 299 (ESMS, MH⁺); ¹ H NMR (CD₃ OD) δ 8.22 & 8.18 (two s, 1H), 4.63 (broad, 1H), 4.30 & 3.60 (two m, 1H), 3.76 (m, 1H), 2.99 (m, 2H), 1.44 (s, 9H), 1.27 (d, 3H, J=6.5 Hz), 1.21 (d, 3H, J=6.5 Hz), 1.91-0.82 (m, 9H).

N-[4-(2-Methoxyethylformylamino)-cyclohexyl]methylcarbamate was similarly prepared: 58% yield; 315 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.25 & 8.16 (two s, 1H), 4.80 (broad, 1H), 4.07 & 3.23 (two m, 1H), 3.50 (m, 2H), 3.40-3.33 (m, 2H), 3.31 (s, 3H), 2.99 (m, 2H), 1.46 (s, 9H), 1.86-0.95 (m, 9H).

N-[4-(Aminomethyl)cyclohexyl]-N-isopropylformamide: Dioxane containing HCl was added (10 ml of 4N HCl solution) to the solution of tert-Butyl N-[4-(isopropylformylamino)cyclohexyl]methylcarbamate dissolved in 10 ml Et₂ O, stirred at room temperature for 2 hours, and the solvent removed to obtain N-[4-(aminomethyl)cyclohexyl]-N-isopropylformamide: 100% yield, 199 (ESMS, MH⁺); ¹ H NMR (CD₃ OD) δ 8.16 (s, 1H), 4.16 & 3.57 (two m, 1H), 3.70 (m, 1H), 2.79 (m, 2H), 1.36 (m, 6H), 1.91-1.06 (m, 9H).

N-[4-(Aminomethyl)cyclohexyl]-N-(2-methoxyethylformamide was similarly obtained: 100% yield; 215 (ESMS, MH⁺); ¹ H NMR (CD₃ OD) δ 8.44 & 8.03 4.65 (two s, 1H), 3.79-3.36 (m, 7H), 3.71 (s, 3H), 2.12-1.13 (m, 9H).

N-Benzoyl-N'-[4-(isopropylformylamino)cyclohexyl]-methylthiourea: N-[4-(Aminomethyl)cyclohexyl]-N-isopropylformamide hydrochloride salt (4.55 mmol, 1 equivalent, obtained from previous step) was stirred at room temperature with benzoyl isothiocyanate (5.46 mmol, 1.2 equivalents) and triethylamine (5.46 mmol, 1.2 equivalents) in THF (50 ml) overnight. Removal of the solvent followed by chromatography afforded a light tan solid: 39% yield, 362 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 10.87 (broad, 1H), 9.20 (broad, 1H), 8.20 & 8.18 (two s, 1H), 7.83 (d, 2H, J=7.7 Hz), 7.60 (m, 1H), 7.49 (m, 2H), 4.26 (m, 1H), 3.76 & 3.08 (two m, 1H), 3.57 (m, 2H), 1.25 (d, 3H, J=6.8 Hz), 1.19 (d, 3H, J=6.8 Hz), 1.97-1.03(m, 9H).

N-Benzoyl-N'-[4-(2-methoxyethylformyl-amino) cyclohexyl]methylthiourea was similarly obtained: 100% yield, 378 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 10.85 (broad, 1H), 9.03 (broad, 1H), 8.18 & 8.08 (two s, 1H), 7.84 (d, 2H, J=7.9 Hz), 7.64 (m, 1H), 7.52 (d, 2H, J=7.8 Hz), 3.63-3.24 (m, 7H), 3.34 & 3.33 (two m, 3H), 2.03-1.13(m, 9H).

N-[4-(Isopropylformylamino)cyclohexyl]methylthiourea: K₂ CO₃ (2 equivalent) was dissolved in 20 ml of water and added to a solution of N-benzoyl-N'-[4-(isopropylformylamino)cyclohexyl]methylthiourea (obtained from the previous step) in MeOH, and the mixture stirred at room temperature overnight. The solvent was removed in vacuo and the residue was dissolved in EtOH. The solution was filtered to remove a white precipitate and the filtrate was concentrated to afford a crude product which was chromatographed to yield the desired material: 100% yield; 258 (ESMS, MH⁺); ¹ H NMR (CD₃ OD) δ 8.15 & 8.13 (two s, 1H), 4.15 & 3.73 (two m, 1H), 3.34 & 2.97 (two m, 1H), 3.29 (m, 2H), 1.26 (d, 3H, J=6.7 Hz), 1.23(d, 3H, J=6.7 Hz), 1.91-1.03 (m, 9H).

N-[4-(2-Methoxyethylformylamino)-cyclohexyl]methylthiourea was similarly prepared: 77% yield, 274 (ESMS, MH⁺); ¹ H NMR (CD₃ OD) δ 8.15 & 8.00 (two s, 1H), 7.55 & 7.43 (two m, 1H), 3.90 & 2.97 (two m, 1H) 3.46-3.28 (m, 10H), 1.90-0.99 (m, 9H).

N-4-[(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]-thiazol-2-ylamino)methyl]cyclohexyl-N-isopropyl-formamide N-[4-(Isopropylformylamino)cyclohexyl]methylthiourea (obtained from the previous step) (0.029 mmol, 1 equivalent) and 4-bromo-2,3,4,5-tetrahydro-1-benzothiepin-5-one (0.044 mmol, 1.5 equivalent) were mixed with 2 equivalents diisopropylethyl amine in 10 ml of EtOH. The resulting mixture was heated at reflux temperature for 2 days. The resulting mixture was concentrated and the crude product was chromatographed (silica) to obtain the desired product. This procedure was used to prepare examples 61-64.

The following examples were prepared according to the reaction sequence of Schemes 1, 2 and 3 which describe the syntheses of sulfonamides, amides and ureas:

EXAMPLE 1

N-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]methanesulfonamide: 74% yield, 413 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.02 (d, 1H, J=7.9 Hz), 7.52 (d, 1H, J=7.8 Hz), 7.33 (apparent t, 1H, J=7.1 Hz), 7.16 (apparent t, 1H, J=6.6 Hz), 5.24 (broad, 1H), 4.38 (broad, 1H), 3.20 (s, 2H), 4.15-3.09 (m, 4H), 2.95, (s, 2H), 1.63 (m, 6H), 1.41 (m, 4H).

EXAMPLE 2

N-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-methanesulfonamide: 819 yield, 424 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.03 (dd, 1H, J=0.7, 7.6 Hz), 7.52 (dd, 1H, J=0.8, 7.6 Hz), 7.33 (apparent dt, 1H, J=0.5, 7.6 Hz), 7.16 (apparent dt, 1H, J=1.3, 7.6 Hz), 4.32 (m, 1H), 3.27 (m, 1H), 3.19 (s, 2H), 3.01 (t, 2H, J=6.5 Hz), 2.96 (s, 3H), 2.08 (ABm, 4H), 1.75-1.46 (m, 4H), 1.32-1.05 (m, 3H).

EXAMPLE 3

N1-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]-1-ethanesulfonamide: 68% yield, 427 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.05 (dd, 1H, J=1.0, 8.4 Hz), 7.53 (dd, 1H, J=0.9, 7.6 Hz), 7.33 (apparent dt, 1H, J=1.3, 7.6 Hz), 7.16 (apparent dt, 1H, J=1.3, 7.6 Hz), 5.06 (m, 1H), 4.05 (m, 1H), 3.26 (m, 2H), 3.20 (s, 2H), 3.11 (m, 2H), 3.03 (q, 2H, J=7.5 Hz), 1.37 (t, 3H, J=7.5 Hz), 1.73-1.32 (m, 10H).

EXAMPLE 4

N1-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]-1-ethanesulfonamide: 87% yield; 480 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.01 (dd, 1H, J=1.6, 7.6 Hz), 7.61-7.57 (m, 2H), 7.52 (dd, 1H, J=0.8, 7.4 Hz), 7.33 (apparent dt, 1H, J=1.5, 7.2 Hz), 7.15 (apparent dt, 1H, J=1.3, 7.2 Hz), 7.09 (dd, 1H, J=3.8, 4.8 Hz), 5.30 (broad, 1H), 4.78 (broad, 1H), 3.23 (broad m, 6H), 3.02 (broad m, 2H), 1.80-1.20 (m, 8H); Anal. Calcd. For C₂₁ H₂₅ N₃ O₂ S₄ +0.15CHCl₃ : C, 51.05; H, 5.43; N, 8.50. Found: C, 51.05; H, 5.09; N, 8.44.

EXAMPLE 5

N1-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-1-ethanesulfonamide: 68% yield, 438 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.05 (dd, 1H, J=1.3, 8.0 Hz), 7.52 (dd, 1H, J=1.0, 7.9 Hz), 7.33 (apparent dt, 1H, J=1.3, 7.6 Hz), 7.16 (apparent dt, 1H, J=1.3, 7.6 Hz), 4.89 (m, 1H), 4.20 (m, 1H), 3.29 (m, 1H), 3.19 (s, 2H), 3.05 (q, 2H, J=7.5 Hz), 2.99 (t, 2H, J=6.4 Hz), 2.09 (ABm, 4H), 1.53 (m, 2H), 1.38 (t, 3H, J=7.5 Hz), 1.17 (m, 5H).

EXAMPLE 6

N2-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-2-thiophenesulfonamide: 58% yield; 492 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.00 (dd, 1H, J=0.9, 7.5 Hz), 7.62-7.59 (m, 2H), 7.52 (dd, 1H, J=7.9, 0.9 Hz), 7.32-7.09 (m, 3H), 5.01 (broad, 1H), 4.76 (broad, 1H), 3.23 (broad m, 5H), 2.88 (t, 2H, J=6.6 Hz), 2.00 (ABm, 4H), 1.70-0.80 (m, 6H); Anal. Calcd. For C₂₂ H₂₅ N₃ O₂ S₄ +0.5H₂ O: C, 52.77; H, 5.23; N, 8.39. Found: C, 53.02; H, 5.02; N, 8.26.

EXAMPLE 7

N1-[5-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]-1-ethanesulfonamide: 55% yield; Anal. Calc. for C₁₈ H₂₆ N₄ S₃ O₂ +0.7 CH₂ Cl₂ : C, 47.68; H, 5.65; N, 8.92. Found: C, 47.89; H, 5.40; N, 8.83; ¹ H NMR (CDCl₃) δ 7.98 (dd, 1H, J=0.6, 7.5 Hz), 7.5 (dd, 1H, J=0.6, 7.5 Hz), 7.30 (t, 1H, J=6.5 Hz), 7.14 (t, 1H, J=6.5 Hz), 6.30 (broad, 1H), 5.50 (broad, 1H), 3.16 (s, 4H), 3.03-2.90 (m, 6H), 1.42-1.20 (m, 9H).

EXAMPLE 8

N2-[5-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]-2-thiophenesulfonamide: 50% yield; Anal. Calc. For C₂₀ H₂₃ N₃ S₃ O₂ +0.20 CH₂ Cl₂ : C, 50.27; H, 4.89; N, 8.71. Found: C, 50.33; H, 4.84; N, 8.47; ¹ H NMR (CDCl₃) δ 7.86 (dd, 1H, J=0.6, 7.5 Hz), 7.60-7.50 (m, 2H), 7.47 (dd, 1H, J=0.6, 7.5 Hz), 7.26-7.04 (m, 3H) 6.22-6.14 (broad, 2H), 3.16 (m, 4H), 3.01 (t, 2H, J=6.5 Hz), 2.83 (t, 2H, J=6.5 Hz), 1.45-1.11 (m, 6H).

EXAMPLE 9

N4-[5-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]-1-methyl-1H-4-imidazolesulfonamide: 45% yield; Anal. Calc. for C₂₀ H₂₅ N₅ S₃ O₂ +0.25 CH₂ Cl₂ : C, 50.16; H, 5.30; N, 14.44. Found: C, 50.04; N, 5.24; H, 14.50; ¹ H NMR (CDCl₃) δ 7.10 (dd, 1H, J=0.6, 7.5 Hz), 7.72 (s, 1H), 7.66 (s, 1H), 7.44 (dd, 1H, J=0.6, 7.5 HZ), 7.31 (m, 1H), 7.147 (t, 1H, J=6.5 Hz), 3.311 (apparent s, 4H), 3.153-3.140 (m, 2H), 3.09 (s, 3H), 2.75 (t, 2H, J=4.5 Hz), 1.48-1.25 (m, 6H).

EXAMPLE 10

N4-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]-2,1,3-benzothiadiazole-4-sulfonamide: 69% yield; 532 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.26 (m, 2H), 8.03 (dd, 1H, J=1.5, 7.5 Hz), 7.73 (dd, 1H, J=6.9, 8.7 Hz), 7.52 (dd, 1H, J=1.5, 7.2 Hz), 7.31 (apparent dt, 1H, J=1.5, 7.2 Hz), 7.15 (apparent dt, 1H, J=1.5, 7.2 Hz), 5.37 (broad, 1H), 5.03 (broad, 1H), 3.33 (m, 6H), 2.92 (apparent q, 2H, J=6.0 Hz), 1.70-1.20 (m, 8H); Anal. Calcd. For C₂₃ H₂₅ N₅ O₂ S₄ +0.5H₂ O: C, 51.09; H, 4.85; N, 12.95. Found: C, 51.09; H, 4.62; H, 12.68.

EXAMPLE 11

N1-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]-2-methoxy-5-methyl-1-benzenesulfonamide: 74% yield; 518 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.04 (dd, 1H, J=1.6, 8.2 Hz), 7.71 (d, 1H, J=1.8 Hz), 7.52 (dd, 1H, J=1.1, 7.8 Hz), 7.35-7.23 (m, 2H), 7.16 (apparent dt, 1H, J=7.2, 1.2 Hz), 6.91 (d, 1H, J=8.4 Hz), 5.08 (broad t, 1H, J=4.7 Hz), 4.88 (t, 1H, J=6.3 Hz), 3.93 (s, 3H), 3.23 (m, 6H), 2.86 (apparent q, 2H, J=6.6 Hz), 2.33 (s, 3H), 1.70-1.20 (m, 8H); Anal. Calcd. For C₂₅ H₃₁ N₃ O₃ N₃ +0.5H₂ O: C, 57.01; H, 6.12; N, 7.98. Found: C, 56.56; H, 5.85; N, 7.56.

EXAMPLE 12

N1-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]-1-naphthalenesulfonamide: 83% yield; 524 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.65 (d, 1H, J=9.2 Hz), 8.26 (dd, 1H, J=1.0, 7.0 Hz), 8.07 (d, 1H, J=8.2 Hz), 8.02 (dd, 1H, J=1.2, 7.7 Hz), 7.97-7.50 (d, 4H), 7.28 (apparent dt, 1H, J=1.3, 7.2 Hz), 7.14 (apparent dt, 1H, J=1.5, 7.2 Hz), 5.13 (broad, 1H), 4.78 (broad, 1H), 3.12 (apparent q, 6H, J=6.0 Hz), 2.89 (apparent q, 2H, J=6.6 Hz), 1.70-1.20 (m, 8H); Anal. Calcd. For C₂₇ H₂₉ N₃ O₂ S₃ +0.4CH₂ Cl₂ : C, 61.50; H, 5.62; N, 7.97. Found: C, 61.42; H, 5.43; N, 7.64.

EXAMPLE 13

N1-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]-5-(dimethylamino)-1-naphthalenesulfonamide: 81% yield; 567 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.64 (d, 1H, J=8.9 Hz), 8.29 (d, 1H, J=8.4 Hz), 8.25 (dd, 1H, J=1.2, 7.4 Hz), 8.02 (dd, 1H, J=1.6, 7.6 Hz), 7.59-7.12 (m, 6H), 3.12 (m, 6H), 2.86 (m, partially covered by singlet, 2H), 2.89 (s, 6H), 1.70-1.20 (m, 8H); Anal. Calcd. For C₂₉ H₃₄ N₄ O₂ S₃ : C, 61.45; H, 6.05; N, 9.88. Found: C, 61.38; H, 6.00; N, 9.50.

EXAMPLE 14

N1-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]-2-nitro-1-benzenesulfonamide: 84% yield; 519 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.15-8.12 (m, 1H), 8.04 (dd, 1H, J=1.6, 8.0 Hz), 7.87-7.84 (m, 1H), 7.74-7.71 (m, 2H), 7.33 (apparent dt, 1H, J=1.3, 7.2 Hz), 7.16 (apparent dt, 1H, J=1.2, 7.2 Hz), 5.30 (broad, 1H), 5.05 (broad, 1H), 3.23 (broad m, 6H), 3.12 (apparent q, 2H, J=6.6 Hz), 1.70-1.20 (m, 8H); Anal. Calcd. For C₂₃ H₂₆ N₄ O₄ S₃ +0.5H₂ O: C, 52.35; H, 5.16; N, 10.62. Found: C, 52.18; H, 4.85; N, 10.14.

EXAMPLE 15

N5-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]-6-chloroimidazo[2,1-b][1,3]thiazole-5-sulfonamide: 68% yield; 554 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.01 (dd, 1H, J=1.1, 7.6 Hz), 7.93 (d, 1H, J=4.6 Hz), 7.52 (dd, 1H, J=1.3, 7.6 Hz), 7.31 (apparent dt, 1H, J=1.2, 7.2 Hz), 7.15 (apparent dt, 1H, J=1.2, 7.2 Hz), 7.03 (d, 1H, J=4.6 Hz), 5.22 (broad, 2H), 3.23 (broad m, 6H), 3.02 (t, 2H, J=6.6 Hz), 1.70-1.20 (m, 8H); Anal. Calcd. For C₂₄ H₂₄ Cl₁ N₅ O₂ S₄ +0.5H₂ O: C, 46.92; H, 4.47; N, 12.44. Found: C, 47.10; H, 4.25; N, 12.18.

EXAMPLE 16

N4-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-2,1,3-benzothiadiazole-4-sulfonamide: 59% yield; 544 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.29-8.24 (m, 2H), 8.03 (dd, 1H, J=1.5, 7.9 Hz), 7.75 (dd, 1H, J=7.0, 8.8 Hz), 7.51 (dd, 1H, J=1.1, 7.8 Hz), 7.32 (apparent dt, 1H, J=1.2, 7.2 Hz), 7.15 (apparent dt, 1H, J=1.2, 7.2 Hz), 5.45 (t, 1H, J=6.9 Hz), 4.87 (broad d, 1H, J=8.1 Hz), 3.23 (broad m, 6H), 2.76 (t, 2H, J=5.7 Hz), 2.01 (ABm, 4H), 1.70-0.80 (m, 5H); Anal. Calcd. For C₂₄ H₂₅ N₅ O₂ S₂ +0.5H₂ O: C, 52.15; H, 4.74; N, 12.67. Found: C, 52.52; H, 4.59; N, 12.36.

EXAMPLE 17

N1-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-2-methoxy-5-methyl-1-benzenesulfonamide: 58% yield; 530 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.03 (dd, 1H, J=1.6, 7.6 Hz), 7.71 (d, 1H, J=1.6 Hz), 7.51 (dd, 1H, J=1.2, 7.8 Hz), 7.35-7.25 (m, 2H), 7.16 (apparent dt, 1H, J=1.2, 7.2 Hz), 6.93 (d, 1, J=8.5 Hz), 5.95 (t, 1H, J=7.2 Hz), 4.86 (d, 1H, J=8.4 Hz), 3.95 (s, 3H), 3.23 (broad m, 5H), 2.71 (t, 2H, J=6.9 Hz), 2.35 (s, 3H), 2.02 (ABm, 4H), 1.70-0.80 (m, 5H); Anal. Calcd. For C₂₆ H₃₁ N₃ O₃ S₃ +0.35CHCl₃ : C, 55.38; H, 5.53; N, 7.35. Found: C, 55.15; H, 5.41; N, 7.13.

EXAMPLE 18

N2-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-5-(2-pyridyl)-2-thiophenesulfonamide: 569 yield; 569 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.60 (dd, 1H, J=5.5 Hz), 8.00 (dd, 1H, J=1.6, 6.6 Hz), 7.80-7.25 (m, 7H), 7.15 (apparent dt, 1H, J=1.2, 7.2 Hz), 5.00 (broad m, 1H), 4.81 (broad m, 1H), 3.23 (broad m, 5H), 2.93 (m, 2H), 2.00 (ABm, 4H), 1.70-0.80 (m, 5H); Anal. Calcd. For C₂₇ H₂₈ N₄ O₂ S₄ : C, 57.01; H, 4.96; N, 9.85. Found: C, 56.60; H, 4.78; N, 9.49.

EXAMPLE 19

N1-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-1-naphthalenesulfonamide: 58% yield; 536 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.65 (d, 1H, J=8.9 Hz), 7.25 (dd, 1H, J=7.3, 0.9 Hz), 8.10 (d, 1H, J=8.2 Hz), 7.98 (apparent dt, 2H, J=0.9, 6.5 Hz), 7.69-7.25 (m, 5H), 7.15 (apparent dt, 1H, J=1.2, 7.2 Hz), 5.00-4.80 (broad, 2H), 3.23 (broad m, 5H), 2.74 (t, 2H, J=6.9 Hz), 2.20-0.80 (m, 9H); Anal. Calcd. For C₂₈ H₂₉ N₃ O₂ S₃ +0.5H₂ O: C, 61.74; H, 5.55; N, 7.71. Found: C, 61.59; H, 5.19; N, 7.47.

EXAMPLE 20

N1-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-5-(dimethylamino)-1-naphthalenesulfonamide: 66% yield; 579 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.56 (d, 1H, J=8.1 Hz), 8.28 (d, 1H, J=8.9 Hz), 8.24 (dd, 1H, J=7.5, 0.9 Hz), 8.01 (dd, 1H, J=8.0, 0.9 Hz), 7.60-7.49 (m, 3H), 7.32-7.10 (m, 3H), 4.87 (d, 1H, J=6.6 Hz), 4.75 (t, 1H, J=5.4 Hz), 3.23 (broad m, 5H), 2.89 (s, 6H), 2.73 (t, 2H, J=6.6 Hz), 1.87 (ABm, 4H), 1.20-0.80 (m, 5H); Anal. Calcd. For C₃₀ H₃₄ N₄ O₂ S₃ +0.5H₂ O: C, 61.30; H, 6.00; N, 9.53. Found: C, 61.16; H, 5.76; N, 9.18.

EXAMPLE 21

N1-[5-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]-5-(dimethylamino)-1 naphthalenesulfonamide: 45% yield; Anal. Calc. for C₂₈ H₃₂ N₄ S₃ O₂ +0.3 CH₃ COOC₂ H₅ : C, 60.55; H, 5.99; N, 9.67. Found: C, 60.60; H, 5.86; N, 9.33; ¹ H NMR (CDCl₃) δ 8.54 (dd, 1H, J=0.6, 7.5 Hz), 8.34 (dd, 1H, J=0.6, 7.5 Hz), 8.22 (dd, 1H, J=0.6, 7.5 Hz), 7.98 (dd, 1H, J=0.6, 7.5 Hz), 7.57-7.49 (m, 3H), 7.26-7.06 (m, 3H), 7.92 (broad, 1H), 5.66 (broad, 1H), 3.13 (apparent s, 4H), 2.94-2.82 (m, 2H), 2.87 (s, 6H), 2.83-2.76 (m, 2H), 1.31-1.04 (m, 6H).

EXAMPLE 22

N1-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-2-nitro-1-benzenesulfonamide: 54% yield; 531 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.15-8.12 (m, 1H), 8.04 (dd, 1H, J=0.9, 7.1 Hz), 7.89-7.76 (m, 2H), 7.76 (dd, 1H, J=0.9, 7.2 Hz), 7.32 (apparent dt, 1H, J=1.2, 7.2 Hz), 7.15 (apparent dt, 1H, J=1.2, 7.2 Hz), 5.36 (broad m, 1H), 4.86 (broad m, 1H), 3.25 (broad m, 5H), 2.96 (t, 2H, J=6.6 Hz), 2.03 (ABm, 4H), 1.70-0.80 (m, 5H); Anal. Calcd. For C₂₄ H₂₆ N₄ O₄ S₃ +0.5H₂ O: C, 53.41; H, 5.04; N, 10.38. Found: C, 53.63; H, 4.72; N, 10.91.

EXAMPLE 23

N4-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-1-methyl-1h-4-imidazolesulfonamide: 28% yield; 490 (ESMS, MH⁺).

EXAMPLE 24

N2-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-5-(3-isoxazolyl)-2-thiophenesulfonamide: 94% yield; 559 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.32 (d, 1H, J=1.8 Hz), 7.98 (dd, 1H, J=8.1, 1.5 Hz), 7.59 (d, 1H, J=3.9 Hz), 7.50 (dd, 1H, J=1.6, 7.8 Hz), 7.46 (d, 1H, J=3.9 Hz), 7.31 (apparent dt, 1H, J=1.2, 7.2 Hz), 7.15 (apparent dt, 1H, J=1.2, 7.2 Hz), 6.53 (d, 1H, J=1.8 Hz), 5.01 (broad, 2H), 3.23 (broad m, 5H), 2.92 (broad m, 2H), 2.02 (ABm, 4H), 1.70-0.80 (m, 5H); Anal. Calcd. For C₂₅ H₂₆ N₄ O₃ S₄ : C, 53.74; H, 4.69; N, 10.03. Found: C, 53.51; H, 4.56; N, 9.56.

EXAMPLE 25

N1-[5-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]-1-naphthalene-sulfonamide: 45% yield; Anal. Calc. for C₂₆ H₂₇ N₃ S₃ O₂ +0.2 CH₃ COOC₂ H₅ : C, 61.04; H, 5.47; N; 9.97. Found: C, 61.35; H, 5.64; N, 7.67; ¹ H NMR (CDCl₃) δ 8.67 (dd, 1H, J=0.6, 7.5 Hz), 8.26 (dd, 1H, J=0.6, 7.5 Hz), 8.05 (dd, 1H, J=0.6, 7.5 Hz), 8.00-7.93 (m, 2H), 7.69-7.48 (m, 4H) 7.19-7.09 (m, 2H), 5.54-5.52 (m, 1H), 5.34-5.29 (m, 1H), 3.18 (apparent s, 4H), 3.02-2.96 (m, 2H), 2.81-2.82 (m, 2H), 1.39-1.08 (m, 6H).

EXAMPLE 26

N1-[5-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]-2-fluoro-1-benzenesulfonamide: 45% yield; Anal. Calc. for C₂₂ H₂₄ FN₃ S₃ O₂ +0.3 CH₃ COOC₂ H₅ : C, 55.28; H, 5.28; N, 8.3. Found: C, 55.43; H, 5.25; N, 8.0. ¹ H NMR (CDCl₃) δ 7.97 (dd, 1H, J=0.6, 7.5 Hz), 7.84 (t, 1H, J=6.5 Hz), 7.58-7.48 (m, 2H), 7.27-7.09 (m, 4H), 6.09-6.08 (m, 1H), 5.69-5.60 (m, 1H), 3.16 (apparent s, 4H), 3.02 (t, 2H, J=6.5 Hz), 2.85 (t, 2H, J=6.5 Hz), 1.45-1.10 (m, 6H).

EXAMPLE 27

N2-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]-5-(3-isoxazolyl)-2-thiophenesulfonamide: 59% yield; 547 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.31 (d, 1H, J=1.9 Hz), 7.98 (dd, 1H, J=1.6, 8.3 Hz), 7.57 (d, 1H, J=4.2 Hz), 7.51 (dd, 1H, J=1.3, 7.8 Hz), 7.44 (d, 1H, J=3.4 Hz), 7.28 (apparent dt, 1H, J=1.2, 7.2 Hz), 7.15 (apparent dt, 1H, J=1.2, 7.2 Hz), 6.51 (d, 1H, J=1.9 Hz), 5.33 (broad, 1H), 5.13 (broad, 1H), 3.23 (broad m, 6H), 3.03 (t, 2H, J=6.6 Hz), 1.80-1.20 (m, 8H); Anal. Calcd. For C₂₄ H₂₆ N₄ O₃ S₄ +1.0H₂ O: C, 51.04; H, 5.00; N, 9.92. Found: C, 50.80; H, 4.69; N, 9.45.

EXAMPLE 28

N1-[5-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]-2-nitro-1-benzenesulfonamide: 40% yield; ¹ H NMR (CDCl₃) δ 8.35-8.25 (m, 1H), 8.05 (d, 1H, J=7.5 Hz), 7.90-7.80 (m, 1H), 7.75-7.70 (m, 1H), 7.55 (d, 1H, J=7.5 Hz), 7.45-7.15 (m, 3H), 5.35-5.25 (m, 1H), 5.10-4.95 (broad, 1H), 3.25-3.10 (m, 6H), 2.40-2.30 (m, 2H), 1.80-1.25 (m, 6H).

EXAMPLE 29

N1-[5-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]-2,6-dichloro-1-benzenesulfonamide: 40% yield; ¹ H NMR (CDCl₃), δ 8.10-8.05 (m, 1H), 8.00 (d, 1H, J=7.5 Hz), 7.50 (d, 1H J=7.5 Hz), 7.48-7.42 (m, 1H), 7.35-7.25 (m, 3H), 5.05 (broad, 1H), 4.1 (broad, 1H), 3.28-3.18 (m, 6H), 3.00-2.90 (m, 2H), 1.75-1.25 (m, 6H).

EXAMPLE 30

N1-[5-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]-2-bromo-6-methoxy-1-benzenesulfonamide: 35% yield; ¹ H NMR (CDCl₃), δ 8.05-7.95 (m, 1H), 7.90-7.85 (m, 1H), 7.65-7.60 (m, 1H), 7.55-7.45 (m, 1H), 7.35-7.18 (m, 2H), 6.90-6.85 (m, 1H), 5.25-5.20 (m, 1H), 4.9 (broad, 1H), 3.95-3.90 (s, 3H), 3.30-3.18 (m, 6H), 2.95-2.85 (m, 2H), 1.75-1.18 (m, 6H).

EXAMPLE 31

N-[5-(4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]phenyl-methanesulfonamide: 40% yield; ¹ H NMR (CDCl₃), δ 8.05-7.95 (m, 2H), 7.65-7.50 (m, 2H), 7.4 (s, 5H), 5.30 (broad, 1H), 4.25 (broad, 1H), 3.30-3.15 (m, 6H), 3.05-2.95 (m, 2H), 2.35-2.25 (m, 2H), 1.80-1.25 (m, 6H).

EXAMPLE 32

N1-[5-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]-2-fluoro-6-methyl-1-benzenesulfonamide: 30% yield; ¹ H NMR (CDCl₃) δ 8.00 (d, 1H, J=7.5 Hz), 7.72-7.65 (m, 2H), 7.52 (d, 1H, J=7.5 Hz), 7.35-7.15 (m, 3H), 5.30 (broad, 1H), 4.65-4.55 (m, 1H), 3.25-3.18 (m, 6H), 3.00-2.90 (m, 2H), 2.60 (s, 3H), 1.82-1.25 (m, 6H).

EXAMPLE 33

N1-[4-(4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)butyl]-2-fluoro-6-methyl-1-benzenesulfonamide: 35% yield; ¹ H NMR (CDCl₃) δ 8.00 (d, 1H, J=7.5 Hz), 7.72-7.65 (m, 2H), 7.52 (d, 1H, J=7.5 Hz), 7.35-7.15 (m, 3H), 5.30 (broad, 1H), 4.85-4.74 (m, 1H), 3.25-3.18 (m, 6H), 3.05-2.95 (m, 2H), 2.6 (s, 3H), 1.82-1.25 (m, 4H).

EXAMPLE 34

N1-[5-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]-1-propanesulfonamide: 30% yield; ¹ H NMR (CDCl₃) δ 8.0 (d, 1H, J=7.5 Hz), 7.5 (d, 1H, J=7.5 Hz), 7.35-7.15 (m, 2H), 3.30-3.22 (m, 6H), 3.15-3.00 (m, 2H), 2.40-2.30 (m, 2H), 1.85-1.20 (m, 6H), 1.10-1.05 (m, 2H), 0.90-0.80 (m, 3H).

EXAMPLE 35

N1-[5-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]-2,4-difluoro-1-benzenesulfonamide: 35% yield; ¹ H NMR (CDCl₃) δ 8.00 (d, 1H, J=7.5 Hz), 7.95-7.85 (m, 1H), 7.50 (d, 1H, J=7.5 Hz), 7.35-7.15 (m, 2H), 6.95-7.05 (m, 2H), 4.82-4.75 (m, 1H), 4.80-4.75 (broad, 1H), 3.28-3.20 (m, 6H), 3.18-3.00 (m, 2H), 1.80-1.20 (m, 6H).

EXAMPLE 36

N1-[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)butyl]-2,4-difluoro-1-benzenesulfonamide: 35% yield; ¹ H NMR (CDCl₃) δ 8.00 (d, 1H, J=7.5 Hz), 7.95-7.85 (m, 1H), 7.50 (d, 1H, J=7.5 Hz), 7.35-7.15 (m, 2H), 6.95-7.05 (m, 1H), 5.15-5.08 (m, 1H), 4.90-4.80 (broad, 1H), 3.30-3.20 (m, 6H), 3.20-3.00 (m, 2H), 1.80-1.20 (m, 4H).

EXAMPLE 37

N'-[5-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]-N,N-dimethylurea: 30% yield; ¹ H NMR (CDCl₃), δ 8.05 (d, 1H, J=7.5 Hz), 7.5 (d, 1H, J=7.5 Hz), 7.42-7.15 (m, 2H), 5.48-5.35 (m, 1H), 4.5-4.4 (broad, 1H), 3.35-3.20 (m, 6H), 2.90 (s, 6H), 1.85-1.18 (m, 6H).

EXAMPLE 38

N1-[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)butyl]-1-naphthamide: 40% yield; ¹ H NMR (CDCl₃), δ 8.32-8.25 (m, 1H), 8.05 (d, 1H, J=7.5 Hz), 7.92-7.85 (m, 2H), 7.60-7.40 (m, 4H), 7.32-7.25 (m, 2H), 7.18-7.10 (m, 1H), 6.20-6.10 (m, 1H), 5.40-5.30 (m, 1H), 3.65-3.55 (m, 2H), 3.40-3.30 (m, 2H), 3.20-3.15 (m, 4H), 1.80-1.18 (m, 4H).

EXAMPLE 39

N2-[5-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]-2-thiophenecarboxamide: 35% yield; ¹ H NMR (CDCl₃) δ 8.05 (d, 1H, J=7.5 Hz), 7.55-7.45 (m, 3H), 7.35-7.28 (m, 1H), 7.20-7.12 (m, 1H), 7.10-7.05 (m, 1H), 6.08-6.02 (m, 1H), 5.30-5.20 (m, 1H), 3.50-3.40 (m, 2H), 3.31-3.22 (m, 1H), 3.20-3.15 (m, 4H), 1.80-1.12 (m, 6H).

EXAMPLE 40

N2-[5-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)pentyl]-2-naphthamide: 30% yield; ¹ H NMR (CDCl₃), δ 8.15 (s, 1H), 8.10 (d, 1H, J=7.5 Hz), 7.95-7.80 (m, 4H), 7.60-7.55 (m, 3H), 7.25-7.22 (m, 1H), 7.18-7.08 (m, 1H), 6.20-6.15 (m, 1H), 5.15-5.10 (m, 1H), 3.55-3.45 (m, 2H), 3.35-3.22 (m, 2H), 3.20-3.15 (m, 4H), 2.20-1.25 (m, 6H).

EXAMPLE 41

N1-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]-1-propanesulfonamide: 10% yield, 440 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.05 (dd, 1H, J=1.6, 8.0 Hz), 7.51 (dd, 1H, J=1.4, 7.9 Hz), 7.33 (apparent dt, 1H, J=1.6, 7.5 Hz), 7.16 (apparent dt, 1H, J=1.4, 8.0 Hz), 5.03 (m, 1H), 4.15 (m, 1H), 3.27 (m, 2H), 3.20 (m, 2H), 3.11 (q, 2H, J=7.1 Hz), 2.98 (t, 2H, J=8.0 Hz), 1.84 (q, 2H,J=7.7), 1.69-1.40 (m, 10H), 1.26 (t, 3H, J=7.1 Hz).

EXAMPLE 42

N1-[6-(4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]-3-(trifluoromethyl)-1-benzenesulfonamide: 18% yield, 542 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.13 (s, 1H), 8.05 (d, 1H, J=8.0 Hz), 8.00 (dd, 1H, J=1.7, 8.0 Hz), 7.84 (dd, 1H, J=0.8, 7.1 Hz), 7.67 (apparent dt, 1H, J=0.5, 8.0 Hz), 7.52 (dd, 1H, J=1.2, 7.5 Hz), 7.30 (apparent dt, 1H, J=1.0, 7.6 Hz), 7.15 (apparent dt, 1H, J=1.2, 7.5 Hz), 5.23 (m, 1H), 4.75 (m, 1H), 3.21 (m, 2H), 3.20 (s, 2H), 2.96 (m, 2H), 1.75-1.28 (m, 10H).

EXAMPLE 43

N1-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]-2,4-difluoro-1-benzenesulfonamide: 14% yield, 510 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.03 (dd, 1H, J=1.6, 7.7 Hz), 7.92 (apparent q, 1H, J=7.7 Hz), 7.52 (dd, 1H, J=1.2, 6.6 Hz), 7.30 (apparent dt, 1H, J=1.6, 7.6 Hz), 7.16 (apparent dt, 1H, J=1.5, 7.6 Hz), 6.99 (m, 2H), 5.07 (m, 1H), 4.72 (m, 1H), 3.23 (m, 2H), 3.20 (s, 1H), 2.98 (m, 2H), 1.62-1.28 (m, 10H).

EXAMPLE 44

N1-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]-2,6-dichloro-1-benzenesulfonamide: 6% yield, 542 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.09 (m, 1H), 8.03 (dm, 1H, J=8.5 Hz), 7.52 (dm, 1H, J=7.7 Hz), 7.47 (m, 2H), 7.36-7.3 (m, 1H), 7.15 (tm, 1H, J=7.2 Hz), 4.98 (b, 1H), 3.30-3.20 (m, 3H), 2.95 (apparent q, 2H, J=7.4 Hz), 1.70-1.20 (m, 12H).

EXAMPLE 45

N1-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]-2-bromo-6-methoxy-1-benzenesulfonamide: 20% yield, 582 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.06-8.03 (m, 2H), 7.62 (dd, 1H, J=2.6, 8.9 Hz), 7.54-7.47 (m, 1H), 7.23 (apparent dt, 1H, J=1.2, 7.2 Hz), 7.15 (apparent dt, 1H, J=1.2, 7.2 Hz), 6.91 (d, 1H, J=9.2 Hz), 4.95 (b, 1H), 4.83 (t, 1H, J=6.6 Hz), 3.95 (s, 3H), 3.23 (m, 2H), 2.90 (apparent q, 2H, J=6.8 Hz), 1.70-1.20 (m, 9H).

EXAMPLE 46

N-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]phenylmethanesulfonamide: 8% yield, 488 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.05 (dd, 1H, J=1.1, 7.8 Hz), 7.48 (dd, 1H, J=1.1, 7.2 Hz), 7.39 (m, 5H), 7.23 (apparent dt, 1H, J=1.2, 7.2 Hz), 7.15 (apparent dt, 1H, J=1.2, 7.2 Hz), 4.98 (b, 1H), 4.55 (s, 2H), 4.03 (b, 1H), 3.25 (m, 2H), 2.97 (m, 2H), 1.70-1.20 (m, 8H).

EXAMPLE 47

N1-[6-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)hexyl]-2-fluoro-6-methyl-1-benzenesulfonamide: 24% yield, 506 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.03 (dd, 1H, J=1.5, 8.0 Hz), 7.69 (dd, 1H, J=2.8, 8.7 Hz), 7.52 (dd, 1H, J=1.3, 7.6 Hz), 7.31 (m, 2H), 7.16 (m, 2H), 5.11 (m, 1H), 4.62 (m, 1H), 3.21 (m, 2H), 3.20 (s, 2H), 2.95 (m, 2H), 2.60 (s, 3H), 1.59-1.25 (m, 10H).

EXAMPLE 48

N1-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-3-(trifluoromethyl)-1-benzenesulfonamide: 12% yield, 554 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.13 (s, 1H), 8.06 (dd, 1H, J=1.0, 7.2 Hz), 8.00 (dd, 1H, J=0.7, 7.3 Hz), 7.86 (dd, 1H, J=1.0, 8.0 Hz), 7.69 (t, 1H, J=7.8 Hz), 7.51 (dd, 1H, J=1.0, 7.6 Hz), 7.30 (t, 1H, J=8.0 Hz), 7.15 (apparent dt, 1H, J=1.0, 7.2 Hz), 4.99 (m, 1H), 4.62 (m, 1H), 3.24 (m, 2H), 3.19 (s, 2H), 2.86 (t, 2H, J=6.4 Hz), 2.00 (ABm, 4H), 1.63-1.03 (m, 6H).

EXAMPLE 49

N1-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-2,4-difluoro-1-benzenesulfonamide: 16% yield, 522 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.03 (dd, 1H, J=1.0, 8.0 Hz), 7.9(m, 1H), 7.51 (dd, 1H, J=1.0,7.7 Hz), 7.32 (apparent dt, 1H, J=1.2, 7.6 Hz), 7.16 (apparent dt, 1H, J=1.3, 7.6 Hz), 7.00 (m, 1H), 4.88 (m, 1H), 4.75 (m, 1H), 3.25 (m, 1H), 3.19 (s, 2H), 2.85 (t, 2H, J=6.5 Hz), 2.05 (ABm, 4H), 1.60-1.45 (m, 4H), 1.26-1.04 (m, 3H).

EXAMPLE 50

N1-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-2,6-dichloro-1-benzenesulfonamide: 18% yield, 554 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.09 (d, 1H, J=1.0, Hz), 8.0 (m, 1H), 7.53-7.48 (m, 3H), 7.32 (apparent dt, 1H, J=0.9, 7.5 Hz), 7.15 (apparent dt, 1H, J=1.5, 7.5 Hz), 5.09 (m, 1H), 4.90 (m, 1H), 3.23 (m, 1H), 3.19 (s, 2H), 2.79 (t, 1H, J=6.4 Hz), 2.04 (ABm, 4H), 1.61 (m, 2H), 1.45 (m, 2H), 1.27-1.03 (m, 3H).

EXAMPLE 51

N-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}phenyl-methanesulfonamide: 4% yield, 500 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.03 (dm, 1H, J=8.1 Hz), 7.51 (dm, 1H, J=8.1 Hz), 7.40 (s, 5H), 7.32 (tm, 1H, J=7.1 Hz), 7.16 (tm, 1H, J=7.1 Hz), 4.93 (b, 1H), 4.26 (s, 2H), 4.09 (b, 1H), 3.24 (b, 2H), 3.19 (s, 2H), 2.85 (t, 2H, J=6.7 Hz), 2.02 (ABm, 4H), 1.70-1.01 (m, 6H).

EXAMPLE 52

N1-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-2-cyano-1-benzenesulfonamide: 16% yield, 511 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.04 (dm, 1H, J=7.8 Hz), 7.93-7.78 (m, 4H), 7.51 (dm, 1H, J=7.3 Hz), 7.35-7.15 (m, 2H), 4.95 (b, 1H), 4.10 (b, 1H), 3.66 (m, 2H), 3.33 (m, 2H), 2.40-1.20 (m, 12H).

EXAMPLE 53

N1-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-4-fluoro-1-benzenesulfonamide: 4% yield, 504 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.02 (dm, 1H, J=8.7 Hz), 7.90-7.85 (m, 2H), 7.51 (dm, 1H, J=7.9 Hz), 7.36-7.16 (m, 4H), 4.86 (b, 1H), 4.42 (b, 1H), 3.30-3.20 (m, 2H), 2.83 (t, 2H, J=6.7 Hz), 2.02 (ABm, 4H), 1.70-0.80 (m, 12H).

EXAMPLE 54

N1-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-4-methyl-1-benzenesulfonamide: 10% yield, 500 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.02 (dd, 1H, J=1.5, 8.0 Hz), 7.41 (d, 1H, J=7.6 Hz), 7.51 (d, 1H, J=7.0 Hz), 7.33-7.28 (m, 3H), 7.15 (apparent dt, 1H, J=1.2, 7.7 Hz), 4.92 (m, 1H), 4.39 (m, 1H), 3.24 (m, 1H), 3.19 (s, 2H), 2.80 (t, 2H, J=6.7 Hz), 2.44 (s, 3H), 2.02 (ABm, 4H), 1.60-1.01 (m, 7H).

EXAMPLE 55

N8-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-8-quinolinesulfonamide: 5390 yield, 537 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 9.04 (dd, 1H, J=1.6,4.2), 8.45 (dd, 1H, J=1.6, 7.4 Hz), 8.31 (dd, 1H, J=1.8, 8.3 Hz), 8.08 (dd, 1H, J=1.3, 8.2 Hz), 8.02 (dd, 1H, J=1.4, 7.9 Hz), 7.68 (t, 1H, J=7.7 Hz), 7.59 (dd, 1H, J=4.1, 8.2 Hz), 7.51 (dd, 1H, J=1.3, 7.7 Hz), 7.31 (apparent dt, 1H, J=1.5, 7.6 Hz), 7.15 (apparent dt, 1H, J=1.5, 7.3 Hz), 6.41 (t, 1H, J=6.1 Hz), 4.89 (broad, 1H), 4.15 (broad, 1H), 3.23 (broad, 1H), 3.18 (s, 2H), 2.71 (t, 2H, J=6.6 Hz), 2.35 (t, 2H, J=7.5 Hz), 1.99 (ABm, 4H), 1.74-0.86 (m, 5H).

EXAMPLE 56

N1-{[4-(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)cyclohexyl]methyl}-2-fluoro-6-methyl-1-benzenesulfonamide: 10% yield, 518 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.04 (d, 1H, J=7.2 Hz), 7.54 (d, 1H, J=5.2 Hz), 7.37-7.26 (m, 4H), 7.16 (tm, 1H, J=7.0 Hz), 4.94 (broad, 1H), 4.59 (broad, 1H), 3.26 (m, 1H), 3.19 (s, 2H), 3.01 (m, 2H), 2.05 (ABM, 4H), 1.45 (s, 3H), 1.63-0.88 (m, 7H).

EXAMPLE 57

N-{5-[(9-Fluoro-4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-yl)amino]pentyl}methanesulfonamide: 45% yield; Anal. Calc. for C₁₇ H₂₂ N₃ S₃ O₂ F : C, 49.2; H, 5.34; N, 10.10. Found: C, 49.35; H, 5.33; N, 9.84; ¹ H NMR (CDCl₃) δ 7.77 (dd, 1H, J=1.1, 7.5 Hz), 7.47 (dd, 1H, J=1.5, 7.5 Hz), 6.87 (m, 1H), 5.46-5.41 (m, 1H), 4.77-4.71 (m, 1H), 3.30-3.00 (m, 8H), 2.96 (s, 3H), 1.76-1.20 (m, 6H).

EXAMPLE 58

N1-{5-[(9-Fluoro-4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-yl)amino]pentyl}-2-methoxy-5-methyl-1-benzenesulfonamide: 55% yield; Anal. Calc. for C₂₄ H₂₈ N₃ FS₃ O₃ : C, 55.26; H, 5.41; N, 8.05. Found: C, 55.18; H, 5.58; N, 7.82; ¹ H NMR (CDCl₃), δ 7.75 (dd, 1H, J=1.1, 7.5 Hz), 7.70 (s, 1H), 7.45 (m, 1H), 7.29 (dd, 1H, J=1.1, 7.5 Hz), 6.94-6.86 (m, 2H), 5.14-5.13 (m, 1H), 4.94-4.98 (m, 1H), 3.93 (s, 3H), 3.26-3.12 (m, 6H), 2.91-2.83 (m, 2H), 2.33 (s, 3H), 1.70-1.13 (m, 6H).

EXAMPLE 59

N1-{5-[(9-Fluoro-4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-yl)amino]pentyl}-2-fluoro-1-benzenesulfonamide: 45% yield; Anal. Calc. for C₂₂ H₂₃ N₃ F₂ S₃ O₂ : C, 53.31; H, 4.68; N, 8.48. Found: C, 53.40; H, 4.87, N, 8.15; ¹ H NMR (CDCl₃) δ 7.92 (t, 1H, J=6.5 Hz), 7.74 (dd, 1H, J=1.1, 7.5 Hz), 7.60-7.53 (m, 1H), 7.47-7.46 (m, 1H), 7.30-7.18 (m, 2H), 6.89-6.83 (m, 1H), 5.43-5.40 (m, 1H), 5.16-5.12 (m, 1H), 3.24-3.12 (m, 6H), 2.99-2.92 (m, 2H), 1.59-1.29 (m, 6H).

EXAMPLE 60

N2-{5-[(9-Fluoro-4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-yl)amino]pentyl}-2-thiophene-sulfonamide: 45% yield; Anal. Calc. for C₂₀ H₂₂ N₃ FS₄ O₂ : C, 49.67; H, 4.58; N, 8.6. Found: C, 49.25; H, 4.67; N, 8.2; M⁺ At 484. ¹ H NMR (CDCl₃), δ 7.74 (dd, 1H, J=1.1, 7.5 Hz), 7.59-7.54 (m, 2H), 7.49-7.44 (m, 1H), 7.09-7.01 (m, 1H), 6.88-6.83 (m, 1H), 5.47-5.44 (m, 1H), 5.06-5.02 (m, 1H), 3.26-3.12 (m, 6H), 3.02-2.96 (m, 2H), 1.60-1.15 (m, 6H).

The following examples were prepared according to Scheme 1b which describes the synthesis of formamides:

EXAMPLE 61

trans-N-4-[(4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)methyl]cyclohexyl-N-(2-methoxyethyl)formamide: 40% yield, 432 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.17 & 8.08 (two s, 1H), 8.01 (dm, 1H, J=8.0 Hz), 7.53 (dm, 1H, J=7.7 Hz), 7.34 (tm, 1H, J=7.5 Hz), 7.17 (dt, 1H, J=1.0, 8.0 Hz), 5.53 (b, 1H), 3.53-3.38 (m, 3H), 3.48 (s, 3H), 3.19 (s, 2H), 3.24-3.07 (m, 4H), 1.98-1.01 (m, 11H).

EXAMPLE 62

trans-N-(4-[(9-Fluoro-4,5-dihydrobenzo[2,3]thiepino-[4,5-d][1,3]thiazol-2-yl)amino]methylcyclohexyl)-N-(2-methoxyethyl)formamide: 24% yield, 450 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.18 & 8.08 (two s, 1H), 7.77 (m, 1H), 7.47 (m, 1H), 6.80 (m, 1H), 5.21(m, 1H), 3.48 (s, 3H), 3.43 (m, 3H), 3.33 (s, 2H), 3.15 (m, 4H), 1.99-1.05 (m, 11H).

EXAMPLE 63

trans-N-4-[(4,5-Dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-ylamino)methyl]cyclohexyl-N-isopropylformamide: 43% yield; 416 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.22 & 8.18 (two s, 1H), 8.03 (dd, 1H, J=1.4, 7.8 Hz), 7.52 (dd, 1H, J=1.5, 8.4 Hz), 7.33 (apparent t, 1H, J=7.0 Hz), 7.16 (apparent dt, 1H, J=1.5, 8.4 Hz), 5.62-5.31 (b, 1H), 3.19 (s, 2H), 3.16 (m, 2H), 3.08 (m, 3H), 1.94-1.54 (m, 7H), 1.23 & 1.20 (two s, 6H), 1.14-1.01 (m, 3H).

EXAMPLE 64

N-(4-[(9-Fluoro-4,5-dihydrobenzo[2,3]thiepino[4,5-d][1,3]thiazol-2-yl)amino]methylcyclohexyl)-N-isopropylformamide: 62% yield, 434 (ESMS, MH⁺); ¹ H NMR (CDCl₃) δ 8.21 & 8.18 (two s, 1H), 7.76 (dd, 1H, J=2.9, 10.7 Hz), 7.47 (m, 1H), 6.87 (m, 1H), 5.52 (m, 1H), 4.29 & 3.60 (two m, 1H), 3.88 (m, 1H), 3.22-3.06 (m, 6H), 1.27 (d, 3H, J=6.9 Hz), 1.21 (d, 3H, J=6.9 Hz), 1.92-0.90 (m, 9H).

II. Synthetic Methods for General Structures

The examples described in Section I are merely illustrative of the methods used to synthesize tricyclic compounds. Further compounds may be obtained utilizing methods shown in Schemes 1-5. The substituents in Schemes 1-5 are described in the Detailed Description.

It may be necessary to incorporate protection and deprotection strategies for substituents such as amino, amido, carboxylic acid, and hydroxyl groups in the synthetic methods described above to form tricyclic derivatives. Methods for protection and deprotection of such groups are well-known in the art, and may be found, for example in Green, T. W. and Wuts, P. G. M. (1991) Protection Groups in Organic Synthesis, 2^(nd) Edition John Wiley & Sons, New York.

III. Oral Compositions

As a specific embodiment of an oral composition of a compound of this invention, 100 mg of one of the compounds described herein is formulated with sufficient finely divided lactose to provide a total amount of 580 to 590 mg to fill a size O hard gel capsule.

IV. Pharmacological Evaluation of Compounds at Cloned Neuropeptide Y-type Receptors

The pharmacological properties of the compounds of the present invention were evaluated at one or more of the cloned human neuropeptide Y-type receptors Y1, Y2, Y4, and Y5, using protocols described below.

Cell Culture

COS-7 cells were grown on 150 mm plates in D-MEM with supplements (Dulbecco's Modified Eagle Medium with 10% bovine calf serum, 4 mM glutamine, 100 units/ml penicillin/100 μg/ml streptomycin) at 37° C., 5% CO₂. Stock plates of COS-7 cells were trypsinized and split 1:6 every 3-4 days. Human embryonic kidney 293 cells were grown on 150 mm plates in D-MEM with supplements (minimal essential medium) with Hanks' salts and supplements (Dulbecco's Modified Eagle Medium with 10% bovine calf serum, 4 mM glutamine, 100 units/ml penicillin/100 μg/ml streptomycin) at 37° C., 5% CO₂. Stock plates of 293 cells were trypsinized and split 1:6 every 3-4 days. Mouse fibroblast LM(tk-) cells were grown on 150 mm plates in D-MEM with supplements (Dulbecco's Modified Eagle Medium with 10% bovine calf serum, 4 mM glutamine, 100 units/mL penicillin/100 μg/mL streptomycin) at 37° C., 5% CO₂. Stock plates of LM(tk-) cells were trypsinized and split 1:10 every 3-4 days.

LM(tk-) cells stably transfected with the human Y5 receptor were routinely converted from an adherent monolayer to a viable suspension. Adherent cells were harvested with trypsin at the point of confluence, resuspended in a minimal volume of complete DMEM for a cell count, and further diluted to a concentration of 10⁶ cells/ml in suspension media (10% bovine calf serum, 10% 10×Medium 199 (Gibco), 9 mM NaHCO₃, 25 mM glucose, 2 mM L-glutamine, 100 units/ml penicillin/100 μg/ml streptomycin, and 0.05% methyl cellulose). The cell suspension was maintained in a shaking incubator at 37° C., 5% CO₂ for 24 hours. Membranes harvested from cells grown in this manner may be stored as large, uniform batches in liquid nitrogen. Alternatively, cells may be returned to adherent cell culture in complete DMEM by distribution into 96-well microtiter plates coated with poly-D-lysine (0.01 mg/ml) followed by incubation at 37° C., 5% CO₂ for 24 hours. Cells prepared in this manner yielded a robust and reliable NPY-dependent response in cAMP radio-immunoassays as further described hereinbelow.

Mouse embryonic fibroblast NIH-3T3 cells were grown on 150 mm plates in Dulbecco's Modified Eagle Medium (DMEM) with supplements (10% bovine calf serum, 4 mM glutamine, 100 units/ml penicillin/100 μg/ml streptomycin) at 37° C., 5% CO₂. Stock plates of NIH-3T3 cells were trypsinized and split 1:15 every 3-4 days.

Sf9 and Sf21 cells were grown in monolayers on 150 mm tissue culture dishes in TMN-FH media supplemented with 10% fetal calf serum, at 27° C., no CO₂. High Five insect cells were grown on 150 mm tissue culture dishes in Ex-Cell 400™ medium supplemented with L-Glutamine, also at 27° C., no CO₂.

Transient Transfection

All receptor subtypes studied (human and rat Y1, human and rat Y2, human and rat Y4, human and rat Y5) were transiently transfected into COS-7 cells by the DEAE-dextran method, using 1 μg of DNA/10⁶ cells (Cullen, 1987). The human Y1 receptor was prepared using known methods (Larhammar, et al., 1992).

Stable Transfection

Human Y1, human Y2, and rat Y5 receptors were co-transfected with a G-418 resistant gene into the human embryonic kidney 293 cell line by a calcium phosphate transfection method (Cullen, 1987). Stably transfected cells were selected with G-418. Human Y4 and human Y5 receptors were similarly transfected into mouse fibroblast LM(tk-) cells and NIH-3T3 cells.

Binding of the compounds of the present invention to human Y1, Y2, Y4, and Y5 receptors was evaluated using stably transfected 293 or LM(tk-) cells as described above. Stably transfected cell lines which may be used for binding assays include, for example, for the human Y1 receptor, 293-hY1-5 (deposited Jun. 4, 1996, under ATCC Accession No. CRL-12121), for the human Y2 receptor, 293-hY2-10 (deposited Jan. 27, 1994, under ATCC Accession No. CRL-11537), for the human Y4 receptor, L-hY4-3 (deposited Jan. 11, 1995, under ATCC Accession No. CRL-11779), and for human Y5 receptor, L-hY5-7 (deposited Nov. 15, 1995, under ATCC Accession No. CRL-11995). These cell lines were deposited with the American Type Culture Collection (ATCC), 10801 University Blvd., Manassas, Va. 20110-2209, U.S.A. under the provisions of the Budapest Treaty for the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure.

Membrane Harvest

Membranes were harvested from COS-7 cells 48 hours after transient transfection. Adherent cells were washed twice in ice-cold phosphate buffered saline (138 mM NaCl, 8.1 mM Na₂ HPO₄, 2.5 mM KCl, 1.2 mM KH₂ PO₄, 0.9 mM CaCl₂, 0.5 mM MgCl₂, pH 7.4) and lysed by sonication in ice-cold sonication buffer (20 mM Tris-HCl, 5 mM EDTA, pH 7.7). Large particles and debris were cleared by low speed centrifugation (200×g, 5 min, 4° C.). Membranes were collected from the supernatant fraction by centrifugation (32,000×g, 18 min, 4° C.), washed with ice-cold hypotonic buffer, and collected again by centrifugation (32,000×g, 18 min, 4° C.). The final membrane pellet was resuspended by sonication into a small volume of ice-cold binding buffer (˜1 ml for every 5 plates: 10 mM NaCl, 20 mM HEPES, 0.22 mM KH₂ PO₄, 1.26 mM CaCl₂, 0.81 mM MgSO₄, pH 7.4). Protein concentration was measured by the Bradford method (Bradford, 1976) using Bio-Rad Reagent, with bovine serum albumin as a standard. Membranes were held on ice for up to one hour and used fresh, or flash-frozen and stored in liquid nitrogen.

Membranes were prepared similarly from 293, LM(tk-), and NIH-3T3 cells. To prepare membranes from baculovirus infected cells, 2×10⁷ Sf21 cells were grown in 150 mm tissue culture dishes and infected with a high-titer stock of hY5BB3. Cells were incubated for 2-4 days at 27° C., no CO₂ before harvesting and membrane preparation as described above.

Membranes were prepared similarly from dissected rat hypothalamus. Frozen hypothalami were homogenized for 20 seconds in ice-cold sonication buffer with the narrow probe of a Virtishear homogenizer at 1000 rpm (Virtis, Gardiner, N.Y.). Large particles and debris were cleared by centrifugation (200×g, 5 min, 4° C.) and the supernatant fraction was reserved on ice. Membranes were further extracted from the pellet by repeating the homogenization and centrifugation procedure two more times. The supernatant fractions were pooled and subjected to high speed centrifugation (100,000×g, 20 min. 4° C.). The final membrane pellet was resuspended by gentle homogenization into a small volume of ice-cold binding buffer (1 mL/gram wet weight tissue) and held on ice for up to one hour, or flash-frozen and stored in liquid nitrogen.

Radioligand Binding to Membrane Suspensions

Membrane suspensions were diluted in binding buffer supplemented with 0.1% bovine serum albumin to yield an optimal membrane protein concentration so that ¹²⁵ I-PYY (or alternative radioligand such as ¹²⁵ I-NPY, ¹²⁵ I-PYY₃₋₃₆, or ¹²⁵ I-[Leu³¹ Pro³⁴ ]PYY) bound by membranes in the assay was less than 10% of ¹²⁵ I-PYY (or alternative radioligand) delivered to the sample (100,000 dpm/sample=0.08 nM for competition binding assays). ¹²⁵ I-PYY (or alternative radioligand) and peptide competitors were also diluted to desired concentrations in supplemented binding buffer. Individual samples were then prepared in 96-well polypropylene microtiter plates by mixing ¹²⁵ I-PYY (25 μL) (or alternative radioligand), competing peptides or supplemented binding buffer (25 μL), and finally, membrane suspensions (200 μL). Samples were incubated in a 30° C. water bath with constant shaking for 120 min. Incubations were terminated by filtration over Whatman GF/C filters (pre-coated with 1% polyethyleneimine and air-dried before use), followed by washing with 5 mL of ice-cold binding buffer. Filter-trapped membranes were impregnated with MeltiLex solid scintillant (Wallac, Turku, Finland) and counted for ¹²⁵ I in a Wallac Beta-Plate Reader. Alternatively, incubations were carried out in GF/C filter plates (pre-coated with 1% polyethyleneimine and air-dried before use), followed by vacuum filtration and three washes of 300 μL of ice-cold binding buffer. 50 μL of UltimaGold (Packard) scintillant were added and counted for ¹²⁵ I in a Wallac MicroBeta Trilux. Non-specific binding was defined by 300 nM human NPY for all receptors except the Y4 subtypes; 100 nM human PP was used for the human Y4 and 100 nM rat PP for the rat Y4. Specific binding in time course and competition studies was typically 80%; most non-specific binding was associated with the filter. Binding data were analyzed using nonlinear regression and statistical techniques available in the GraphPAD Prism package (San Diego, Calif.).

Functional Assay: Radioimmunoassay of cAMP

Stably transfected cells were seeded into 96-well microtiter plates and cultured until confluent. To reduce the potential for receptor desensitization, the serum component of the media was reduced to 1.5% for 4 to 16 hours before the assay. Cells were washed in Hank's buffered saline, or HBS (150 mM NaCl, 20 mM HEPES, 1 mM CaCl₂, 5 mM KCl, 1 mM MgCl₂, and 10 mM glucose) supplemented with 0.1% bovine serum albumin plus 5 mM theophylline and pre-equilibrated in the same solution for 20 min at 37° C. in 5% CO₂. Cells were then incubated 5 min with 10 μM forskolin and various concentrations of receptor-selective ligands. The assay was terminated by the removal of HBS and acidification of the cells with 100 mM HCl. Intracellular cAMP was extracted and quantified with a modified version of a magnetic bead-based radioimmunoassay (Advanced Magnetics, Cambridge, Mass.). The final antigen/antibody complex was separated from free ¹²⁵ I-cAMP by vacuum filtration through a PVDF filter in a microtiter plate (Millipore, Bedford, Mass.). Filters were punched and counted for ¹²⁵ I in a Packard gamma counter. Binding data were analyzed using nonlinear regression and statistical techniques available in the GraphPAD Prism package (San Diego, Calif.).

Functional Assay: Intracellular calcium mobilization

The intracellular free calcium concentration was measured by microspectroflourometry using the fluorescent indicator dye Fura-2/AM. Stably transfected cells were seeded onto a 35 mm culture dish containing a glass coverslip insert. Cells were washed with HBS and loaded with 100 μl of Fura-2/AM (10 μM) for 20 to 40 min. After washing with HBS to remove the Fura-2/AM solution, cells were equilibrated in HBS for 10 to 20 min. Cells were then visualized under the 40× objective of a Leitz Fluovert FS microscope and fluorescence emission was determined at 510 nM with excitation wave lengths alternating between 340 nM and 380 nM. Raw fluorescence data were converted to calcium concentrations using standard calcium concentration curves and software analysis techniques.

Materials

Cell culture media and supplements were from Specialty Media (Lavallette, N.J.). Cell culture plates (150 mm and 96-well microtiter) were from Corning (Corning, N.Y.). Sf9, Sf21, and High Five insect cells, as well as the baculovirus transfer plasmid, pBlueBacIII™, were purchased from Invitrogen (San Diego, Calif.). TMN-FH insect medium complemented with 10% fetal calf serum, and the baculovirus DNA, BaculoGold™, was obtained from Pharmingen (San Diego, Calif.). Ex-Cell 400™ medium with L-Glutamine was purchased from JRH Scientific. Polypropylene 96-well microtiter plates were from Co-star (Cambridge, Mass.). All radioligands were from New England Nuclear (Boston, Mass.). Commercially available NPY and related peptide analogs were either from Bachem California (Torrance, Calif.) or Peninsula (Belmont, Calif.); [D-Trp³² ]NPY and PP C-terminal fragments were synthesized by custom order from Chiron Mimotopes Peptide Systems (San Diego, Calif.). Bio-Rad Reagent was from Bio-Rad (Hercules, Calif.). Bovine serum albumin (ultra-fat free, A-7511) was from Sigma (St. Louis. Mo.). All other materials were reagent grade.

Radioligand Binding Assay Results

The compounds described above were assayed using cloned human NPY receptors. The preferred compounds were found to be selective NPY (Y5) antagonists. The binding affinities of several compounds for NPY (Y5) are illustrated in Table 1.

                                      TABLE 1                                      __________________________________________________________________________     EXAMPLE                                       K.sub.i, nM                                                                        K.sub.i, nM                  No.   STRUCTURE                               hNPY-5                                                                             hNPY-1,2,4                   __________________________________________________________________________            ##STR14##                              7.4                              2                                                                                     ##STR15##                              6.8                              3                                                                                     ##STR16##                              5.4                              4                                                                                     ##STR17##                              2.9 >10000                       5                                                                                     ##STR18##                              5.1 >10000                       6                                                                                     ##STR19##                              5.1                              7                                                                                     ##STR20##                              3.7 >10000                       8                                                                                     ##STR21##                              2.6 >10000                       9                                                                                     ##STR22##                              17.2                             10                                                                                    ##STR23##                              4.4                              11                                                                                    ##STR24##                              5.4                              12                                                                                    ##STR25##                              16.6                             13                                                                                    ##STR26##                              71                               14                                                                                    ##STR27##                              7.1                              15                                                                                    ##STR28##                              6.6                              16                                                                                    ##STR29##                              2.4 >10000                       17                                                                                    ##STR30##                              14.1                             18                                                                                    ##STR31##                              54                               19                                                                                    ##STR32##                              18.4                             20                                                                                    ##STR33##                              27                               21                                                                                    ##STR34##                              161                              22                                                                                    ##STR35##                              11.5                             23                                                                                    ##STR36##                              33                               24                                                                                    ##STR37##                              34                               25                                                                                    ##STR38##                              17.2                             26                                                                                    ##STR39##                              3.7                              27                                                                                    ##STR40##                              29                               28                                                                                    ##STR41##                              5.2                              29                                                                                    ##STR42##                              71                               30                                                                                    ##STR43##                              9.7                              31                                                                                    ##STR44##                              38                               32                                                                                    ##STR45##                              8.3                              33                                                                                    ##STR46##                              110                              34                                                                                    ##STR47##                              24                               35                                                                                    ##STR48##                              6.5                              36                                                                                    ##STR49##                              119                              37                                                                                    ##STR50##                              122                              38                                                                                    ##STR51##                              123                              39                                                                                    ##STR52##                              84                               40                                                                                    ##STR53##                              100                              41                                                                                    ##STR54##                              3.6                              42                                                                                    ##STR55##                              22.4                             43                                                                                    ##STR56##                              4.1                              44                                                                                    ##STR57##                              25                               45                                                                                    ##STR58##                              7.9                              46                                                                                    ##STR59##                              10.5                             47                                                                                    ##STR60##                              4                                48                                                                                    ##STR61##                              21                               49                                                                                    ##STR62##                              7.9                              50                                                                                    ##STR63##                              17.4                             51                                                                                    ##STR64##                              8.9                              52                                                                                    ##STR65##                              69                               53                                                                                    ##STR66##                              9.1                              54                                                                                    ##STR67##                              6.6                              55                                                                                    ##STR68##                              5.7                              56                                                                                    ##STR69##                              8.2 >10000                       57                                                                                    ##STR70##                              6.1 >10000                       58                                                                                    ##STR71##                              2.8 >10000                       59                                                                                    ##STR72##                              4.9 >10000                       60                                                                                    ##STR73##                              4.8 >10000                       61                                                                                    ##STR74##                              12.3                             62                                                                                    ##STR75##                              13                               63                                                                                    ##STR76##                              4.8                              64                                                                                    ##STR77##                              6                                __________________________________________________________________________      ##STR78##      Functional Assay Results

The functional in vitro activity of several compounds were characterized using a radioimmunoassay of cAMP, the results of which are summarized in Table 2.

                  TABLE 2                                                          ______________________________________                                         Functional Antagonism Data                                                     Example #     K.sub.i (h NPY-5), nM                                                                      pK.sub.b                                             ______________________________________                                         2             6.8         8.6                                                  55            5.7         7.7                                                  ______________________________________                                    

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What is claimed is:
 1. A compound having the structure: ##STR79## wherein each R₁ is independently H, F, Cl, Br, --CN, --OH, --NO₂, --NR₅ R₆, --SO₂ R₅, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) NR₅ COR₅, perfluoroalkyl, polyfluoroalkyl, aminoalkyl, or straight chained or branched C₁ -C₇ alkyl;wherein R₅ is independently H; or straight chained or branched C₁ -C₇ alkyl; wherein R₆ is independently H; or straight chained or branched C₁ -C₇ alkyl; wherein B is O, NH or S; wherein X is S, SO or SO₂ ; wherein each n independently is an integer from 0 to 6 inclusive; wherein R₈ is ##STR80## wherein Y is C or N; wherein R₇ is independently straight chained or branched C₁ -C₇ alkyl; wherein R₉ is independently H; or straight chained or branched C₁ -C₄ alkyl; wherein R₁₀ is independently H; or straight chained or branched C₁ -C₄ alkyl; wherein R₁₁ is ##STR81## wherein R₁₂ is H, straight chained or branched C₁ -C₇ alkyl, (CH₂)_(u) OR₁₇, or O(CH₂)_(u) OR₁₇ ; provided that when X is O, R₁₂ cannot be methyl; wherein R₁₃ is independently H; --(CH₂)_(u) OR₅ ; --(CH₂)_(t) CONR₅ R₆ ; --(CH₂)_(u) NR₅ COR₅ ; --(CH₂)_(t) COR₇ ; --(CH₂)_(t) CO₂ R₅ ; --(CH₂)_(u) NR₅ R₆ ; --(CH₂)_(u) CN; straight chained or branched C₁ -C₇ alkyl; C₁ -C₇ alkyl in which the C₂ -C₇ atoms may be optionally substituted with one or more F or Cl; C₃ -C₇ cycloalkyl-C₁ -C₇ alkyl; straight chained or branched C₂ -C₇ alkenyl or alkynyl; or C₃ -C₇ cycloalkyl; phenyl or C₁ -C₆ phenylalkyl; wherein the phenyl or C₁ -C₆ phenylalkyl may be substituted with one or more of F, Cl, --CN, --NO₂, --NR₅ R₆, --SO₂ R₅, --(CH₂)_(n) COR₇, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) NR₅ COR₅, --(CH₂)_(n) CO₂ R₅, --(CH₂)_(n) SO₂ NR₅ R₆, straight chained or branched C₁ -C₇ alkyl, perfluoroalkyl, polyfluoroalkyl, or aminoalkyl; or R₁₂ and R₁₃ together with the amide linkage to which they are attached are pyrrolidinonyl, piperidonyl, or oxazolidinonyl; wherein R₁₄ is H; straight chained or branched C₁ -C₄ alkyl; F; or --(CH₂)_(r) OR₅ ; wherein R₁₅ is H, straight chained or branched C₁ -C₄ alkyl, or F; with the proviso that when R₁₄ is --OH, R₁₅ cannot be F; wherein R₁₆ is perfluoroalkyl, unsubstituted straight chained or branched C₁ -C₇ alkyl, substituted straight chained or branched C₂ -C₇ alkyl, wherein the C₂ -C₇ alkyl may be substituted with one or more of F, Cl, --CN, --SO₂ R₅, --(CH₂)_(n) COR₇, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) NR₅ COR₅, --(CH₂)_(n) CO₂ R₅, --(CH₂)_(n) OCF₃, perfluoroalkyl, polyfluoroalkyl, or aminoalkyl, straight chained or branched C₂ -C₇ alkenyl or alkynyl, or C₃ -C₇ cycloalkyl or cycloalkenyl; C₃ -C₇ cycloalkyl or cycloalkenyl; phenyl, heteroaryl, or C₁ -C₇ phenylalkyl, wherein the phenyl, heteroaryl, or C₁ -C₇ phenylalkyl may be substituted with one or more of F, Cl, Br, --CN, --NO₂, --NR₅ R₆, --(CH₂)_(n) NR₅ COR₅, --SO₂ R₅, --(CH₂)_(n) COR₇, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) CO₂ R₅, --(CH₂)_(n) SO₂ NR₅ R₆, ethylenedioxy, methylenedioxy, straight chained or branched C₁ -C₇ alkyl, perfluoroalkyl, polyfluoroalkyl, or aminoalkyl, straight chained or branched C₂ -C₇ alkenyl or alkynyl, or C₃ -C₇ cycloalkyl or cycloalkenyl; quinolinyl, 1-naphthyl, 2-naphthyl, or 2,1,3-benzothiadiazolyl; wherein the quinolinyl, 1-naphthyl, 2-naphthyl or 2,1,3-benzothiadiazolyl may be substituted with one or more of F, Cl, Br, --CN, --NO₂, --NR₅ R₆, --(CH₂)_(n) NR₅ COR₅, --SO₂ R₅, --(CH₂)_(n) COR₇, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) CO₂ R₅, --(CH₂)_(n) SO₂ NR₅ R₆, ethylenedioxy, methylenedioxy, straight chained or branched C₁ -C₇ alkyl, perfluoroalkyl, polyfluoroalkyl, or aminoalkyl; with the proviso that when R₈ is NR₉ (R₁₄ R₁₅)_(s) NR₁₀ R₁₁, R₁₆ cannot be quinolinyl; wherein R₁₇ is H, straight chained or branched C₁ -C₄ alkyl, perfluoroalkyl, or polyfluoroalkyl; wherein R₁₉ is --(CH₂)_(u) OR₅, --NR₅ R₆, phenyl, or heteroaryl, wherein the phenyl or heteroaryl may be substituted with one or more of F, Cl, Br, --CN, --NO₂, --NR₅ R₆, --(CH₂)_(n) NR₅ COR₅, --SO₂ R₅, --(CH₂)_(n) COR₇, --(CH₂)_(n) OR₅, --(CH₂)_(n) CONR₅ R₆, --(CH₂)_(n) CO₂ R₅, --(CH₂)_(n) SO₂ NR₅ R₆, ethylenedioxy, methylenedioxy, straight chained or branched C₁ -C₇ alkyl, perfluoroalkyl, polyfluoroalkyl, or aminoalkyl, straight chained or branched C₂ -C₇ alkenyl or alkynyl, or C₃ -C₇ cycloalkyl or cycloalkenyl; wherein m is 0 or 1; wherein each p independently is an integer from 0 to 2 inclusive; wherein each r independently is an integer from 0 to 3 inclusive; wherein each s independently is an integer from 1 to 6 inclusive; wherein t is an integer from 1 to 4 inclusive; wherein each u independently is an integer from 2 to 4 inclusive; wherein v is 1 or 2; with the proviso that when v is 2, m is 0; wherein z is an integer from 2 to 7; or a pharmaceutically acceptable salt thereof.
 2. The compound of claim 1, wherein the compound comprises the (+) enantiomer.
 3. The compound of claim 1, wherein the compound comprises the (-) enantiomer.
 4. The compound of claim 1 having the structure: ##STR82##
 5. The compound of claim 4 having the structure:
 6. The compound of claim 5 having the structure:
 7. The compound of claim 4 having the structure:
 8. The compound of claim 7 selected from the group consisting of:
 9. The compound of claim 4 having the structure:
 10. The compound of claim 9 having the structure:
 11. A pharmaceutical composition comprising a therapeutically effective amount of the compound of claim 1 and a pharmaceutically acceptable carrier.
 12. A pharmaceutical composition of claim 11, wherein the amount of the compound is an amount from about 0.01 mg to about 800 mg.
 13. A pharmaceutical composition of claim 12, wherein the amount of the compound is an amount from about 0.01 mg to about 500 mg.
 14. A pharmaceutical composition of claim 13, wherein the amount of the compound is an amount from about 0.01 mg to about 250 mg.
 15. A pharmaceutical composition of claim 14, wherein the amount of the compound is an amount from about 0.1 mg to about 60 mg.
 16. A pharmaceutical composition of claim 15, wherein the amount of the compound is an amount from about 1 mg to about 20 mg.
 17. The pharmaceutical composition of claim 11, wherein the carrier is a liquid and the composition is a solution.
 18. The pharmaceutical composition of claim 11, wherein the carrier is a solid and the composition is a tablet.
 19. The pharmaceutical composition of claim 11, wherein the carrier is a gel and the composition is a suppository.
 20. A pharmaceutical composition made by combining a therapeutically effective amount of the compound of claim 1 and a pharmaceutically acceptable carrier.
 21. A process for making a pharmaceutical composition comprising combining a therapeutically effective amount of the compound of claim 1 and a pharmaceutically acceptable carrier. 